Efficient single-copy HDR by 5’ modified long dsDNA donors
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.39468
Abstract: CRISPR/Cas9 efficiently induces targeted mutations via non-homologous-end-joining but for genome editing, precise, homology-directed repair (HDR) of endogenous DNA stretches is a prerequisite. To favor HDR, many approaches interfere with the repair machinery or manipulate Cas9… read more here.
Keywords: copy hdr; efficient single; single copy; long dsdna ... See more keywords