Articles with "loop mediated" as a keyword



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Assessment of a Smartphone-Based Loop-Mediated Isothermal Amplification Assay for Detection of SARS-CoV-2 and Influenza Viruses

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Published in 2022 at "JAMA Network Open"

DOI: 10.1001/jamanetworkopen.2021.45669

Abstract: Key Points Question Can loop-mediated isothermal amplification (LAMP)-based methodology coupled with smartphone detection provide an inexpensive, rapid, sensitive, and reliable platform for COVID-19 and influenza testing? Findings In this cohort study of saliva samples from… read more here.

Keywords: sars cov; loop mediated; isothermal amplification; smartphone based ... See more keywords
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Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS‐CoV‐2 by Reverse Transcription Loop‐Mediated Isothermal Amplification

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Published in 2023 at "Advanced Healthcare Materials"

DOI: 10.1002/adhm.202202972

Abstract: Quantitative polymerase chain reaction (qPCR) is considered the gold standard for pathogen detection. However, improvement is still required, especially regarding the possibilities of decentralization. Apart from other reasons, infectious diseases demand on‐site analysis to avoid… read more here.

Keywords: detection sars; transcription loop; detection; loop mediated ... See more keywords
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Loop-mediated isothermal amplification (LAMP) as an alternative to PCR: A rapid on-site detection of gene doping.

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Published in 2017 at "Drug testing and analysis"

DOI: 10.1002/dta.2324

Abstract: Innovation in medical research has been diverted at multiple occasions to enhance human performance. The predicted great progress in gene therapy has raised some concerns regarding its misuse in the world of sports (gene doping)… read more here.

Keywords: loop mediated; mediated isothermal; detection; isothermal amplification ... See more keywords
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Rapid detection of the New Delhi metallo‐b‐lactamase 1 (NDM‐1) gene by loop‐mediated isothermal amplification (LAMP)

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Published in 2018 at "Journal of Clinical Laboratory Analysis"

DOI: 10.1002/jcla.22323

Abstract: New Delhi Metallo‐b‐lactamase (NDM‐1) is an enzyme emerging around the world conferring resistance to a wide range of β‐lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the… read more here.

Keywords: loop mediated; detection; new delhi; lactamase ndm ... See more keywords
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Diagnostic value of loop‐mediated isothermal amplification assay for hand, foot, and mouth disease

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Published in 2021 at "Journal of Clinical Laboratory Analysis"

DOI: 10.1002/jcla.23776

Abstract: Nowadays, hand, foot, and mouth disease (HFMD) has a significant negative impact on children's health, especially in the Asia‐Pacific region. Loop‐mediated isothermal amplification assay (LAMP) is a highly efficient and convenient novel tool. However, its… read more here.

Keywords: loop mediated; mediated isothermal; foot mouth; isothermal amplification ... See more keywords
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Detection of SARS‐CoV‐2 in different human biofluids using the loop‐mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil

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Published in 2022 at "Journal of Medical Virology"

DOI: 10.1002/jmv.27842

Abstract: We adopted the reverse‐transcriptase‐loop‐mediated isothermal amplification (RT‐LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS‐Cov‐2) in patient samples. Two primer sets for genes N and Orf1ab were designed to detect SARS‐CoV‐2, and one primer… read more here.

Keywords: isothermal amplification; mediated isothermal; loop mediated; sars cov ... See more keywords
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Rapid point‐of‐care diagnostics for the detection of Chlamydia pecorum in koalas (Phascolarctos cinereus) using loop‐mediated isothermal amplification without nucleic acid purification

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Published in 2019 at "MicrobiologyOpen"

DOI: 10.1002/mbo3.916

Abstract: Infectious disease, predominately chlamydiosis, contributes significantly to the decline in health of wild koala (Phascolarctos cinereus) populations in some regions of Australia. In this study, we describe the development and evaluation of a simple, sensitive,… read more here.

Keywords: loop mediated; phascolarctos cinereus; mediated isothermal; detection ... See more keywords
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A loop-mediated isothermal amplification (LAMP) assay for detection of Toxoplasma gondii infection in women with spontaneous abortion

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Published in 2020 at "Archives of Microbiology"

DOI: 10.1007/s00203-020-02081-w

Abstract: The present study aimed to use the loop-mediated isothermal amplification (LAMP) technique in comparison with serological tests to determine the rate of  T. gondii infection in women suffering from spontaneous abortion (SA). A total of… read more here.

Keywords: loop mediated; amplification lamp; mediated isothermal; gondii infection ... See more keywords
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Dual-modality loop-mediated isothermal amplification for pretreatment-free detection of Septin9 methylated DNA in colorectal cancer

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Published in 2021 at "Mikrochimica Acta"

DOI: 10.1007/s00604-021-04979-8

Abstract: Currently, the determination of DNA methylation is still a challenge due to the limited efficiency of enrichment, bisulfite modification, and detection. In this study, a dual-modality loop-mediated isothermal amplification integrated with magnetic bead isolation is… read more here.

Keywords: loop mediated; mediated isothermal; detection; colorectal cancer ... See more keywords
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Development of loop-mediated isothermal amplification assays for rapid detection of blackleg pathogens in Swedish winter oil seed rape

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Published in 2020 at "European Journal of Plant Pathology"

DOI: 10.1007/s10658-020-02004-x

Abstract: Blackleg is a devastating disease of winter oil seed rape (WOSR) caused by the two species Leptosphaeria maculans and L. biglobosa “brassicae”. In this study, Loop-Mediated Isothermal Amplification (LAMP) assays were developed using two primer… read more here.

Keywords: loop mediated; seed; mediated isothermal; seed rape ... See more keywords
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Detection of high-molecular-weight glutenin subunit genes for 1Dx2 and 1Dx5 using loop-mediated isothermal amplification assay

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Published in 2017 at "Molecular Breeding"

DOI: 10.1007/s11032-017-0702-0

Abstract: High-molecular-weight glutenin subunits (HMW-GS) in wheat grain are the major determinants of dough elasticity and viscosity and thus of bread-making quality. PCR-based molecular markers designed based on DNA polymorphisms were used to analyze HMW-GS genes… read more here.

Keywords: loop mediated; 1dx2 1dx5; detection; high molecular ... See more keywords