Teicoplanin aglycone media and carboxypeptidase Y: Tools for finding low-abundance D-amino acids and epimeric peptides.
Sign Up to like & getrecommendations! Published in 2023 at "Chirality"
DOI: 10.1002/chir.23543
Abstract: D-amino acids and epimeric peptides/proteins can play crucial biological roles and adversely affect protein folding and oligopeptide aggregation in age-related pathologies in humans. This has ignited interest in free D-amino acids as well as those… read more here.
Keywords: acids epimeric; amino acids; teicoplanin aglycone; epimeric peptides ... See more keywords
Evaluation of the Potential Risk of Advanced Peak Determination in Distorting Isobaric Labeling‐Based Single‐Shot Proteome Quantitation
Sign Up to like & getrecommendations! Published in 2020 at "PROTEOMICS"
DOI: 10.1002/pmic.201900255
Abstract: The recent development and implementation of the advanced peak determination (APD) algorithm with MS instrument dramatically increased the sampling of low abundance features for MS/MS fragmentation. After in‐depth evaluation, it is found that with APD… read more here.
Keywords: advanced peak; single shot; abundance; peak determination ... See more keywords
Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope™ in Cultured Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_7
Abstract: Detecting low-abundance long noncoding RNAs (lncRNAs) is extremely difficult due to their expression levels. Deeper sequencing with extensive protocols is required to detect these RNAs and high-throughput screens to examine the regulation of these RNAs… read more here.
Keywords: long noncoding; abundance; using rnascope; low abundance ... See more keywords
Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_14
Abstract: Immunocytochemistry can be instrumental in assessing the spatial distribution and relative levels of epigenetic modifications. Although conventional immunostaining has been utilized for the detection of 5-methylcytosine (5mC) in animal cells and tissues for several decades,… read more here.
Keywords: dna; immunocytochemistry; detection low; low abundance ... See more keywords
Detection of low-abundance point mutations by competitive strand assisted endonuclease IV signal amplification system
Sign Up to like & getrecommendations! Published in 2017 at "Current Medical Science"
DOI: 10.1007/s11596-017-1808-7
Abstract: SummaryGenetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely… read more here.
Keywords: detection; mutations competitive; point mutations; detection low ... See more keywords
Novel low-abundance allergens from mango via combinatorial peptide libraries treatment: A proteomics study.
Sign Up to like & getrecommendations! Published in 2018 at "Food chemistry"
DOI: 10.1016/j.foodchem.2018.06.113
Abstract: Mango allergy is a rare condition, which may cause severe hypersensitivity reactions, such as anaphylaxis, angioedema, asthma and contact dermatitis. By exploiting the combinatorial peptide ligand library (CPLL) technology, mango proteomes have been extracted and… read more here.
Keywords: mango; allergens mango; novel low; combinatorial peptide ... See more keywords
Efficient extraction of low-abundance peptides from digested proteins and simultaneous exclusion of large-sized proteins with novel hydrophilic magnetic zeolitic imidazolate frameworks.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.02.038
Abstract: Zeolitic imidazolate frameworks (ZIFs) are composed of tetrahedral transition metal ions and imidazolate type organic linkers. This kind of metal organic frameworks (MOFs) combines the unique properties of both zeolites and MOFs. Furthermore, ZIFs can… read more here.
Keywords: hydrophilic magnetic; imidazolate frameworks; abundance peptides; low abundance ... See more keywords
Digital and Absolute Assays for Low Abundance Molecular Biomarkers.
Sign Up to like & getrecommendations! Published in 2023 at "Accounts of chemical research"
DOI: 10.1021/acs.accounts.3c00030
Abstract: ConspectusThere has been a recent surge of advances in biomolecular assays based on the measurement of discrete molecular targets as opposed to signals averaged across molecular ensembles. Many of these "digital" assay designs derive from… read more here.
Keywords: digital absolute; assays low; absolute assays; abundance molecular ... See more keywords
All-Ion Monitoring-Directed Low-Abundance Protein Quantification Reveals CALB2 as a Key Promoter in Hepatocellular Carcinoma Metastasis.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03562
Abstract: Because of the wide abundance range of the proteome, achieving high-coverage quantification of low-abundance proteins is always a major challenge. In this study, a complete pipeline focused on all-ion monitoring (AIM) is first constructed with… read more here.
Keywords: metastasis; quantification; abundance; low abundance ... See more keywords
Ultraspecific Multiplexed Detection of Low-Abundance Single-Nucleotide Variants by Combining a Masking Tactic with Fluorescent Nanoparticle Counting.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b00685
Abstract: To be able to detect simultaneously multiple single-nucleotide variants (SNVs) with both ultrahigh specificity and low-abundance sensitivity is of pivotal importance for molecular diagnostics and biological research. In this contribution, we for the first time… read more here.
Keywords: detection; multiplexed detection; single nucleotide; nucleotide variants ... See more keywords
Light-Activated Nanodevice for On-Demand Imaging of miRNA in Living Cells via Logic Assembly.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c00376
Abstract: Low-abundance biomarker amplification detection systems have been widely used to detect miRNAs; however, "always active" systems are insufficient for high spatial and temporal control of miRNAs. Here, we constructed a light-activated nanodevice (LAN) based on… read more here.
Keywords: activated nanodevice; demand imaging; living cells; demand ... See more keywords