Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8695-8_7
Abstract: Two-dimensional electrophoresis as a complementary approach to gel-free proteomic methods possesses the ability to separate physiologically important isoforms of proteins in an unbiased manner. Frequently, those isoforms are low-abundant regulators, and therefore, detection and identification… read more here.
Keywords: abundant proteins; identification low; low abundant; detection identification ... See more keywords
Detection of Low-Abundant MicroRNAs with Hybridization Microchips
Sign Up to like & getrecommendations! Published in 2019 at "Bulletin of Experimental Biology and Medicine"
DOI: 10.1007/s10517-019-04441-z
Abstract: The effect of low concentrations of miRNA on the ability of GeneChip miRNA 4.0 hybridization chips to evaluate their representation in the sample was studied. It is shown that the evaluation of the expression of… read more here.
Keywords: micrornas hybridization; low abundant; hybridization; abundant micrornas ... See more keywords
Depleting high-abundant and enriching low-abundant proteins in human serum: An evaluation of sample preparation methods using magnetic nanoparticle, chemical depletion and immunoaffinity techniques.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.03.091
Abstract: The efficiency of three different depletion methods to remove the most abundant proteins, enriching those human serum proteins with low abundance is checked to make more efficient the search and discovery of biomarkers. These methods… read more here.
Keywords: abundant proteins; immunoaffinity; low abundant; human serum ... See more keywords
Spectral Clustering Improves Label-Free Quantification of Low-Abundant Proteins
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.8b00377
Abstract: Label-free quantification has become a common-practice in many mass spectrometry-based proteomics experiments. In recent years, we and others have shown that spectral clustering can considerably improve the analysis of (primarily large-scale) proteomics data sets. Here… read more here.
Keywords: data sets; spectral clustering; low abundant; free quantification ... See more keywords
Low-Abundant Microorganisms: The Human Microbiome’s Dark Matter, a Scoping Review
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2021.689197
Abstract: Research on the human microbiome has mainly been restricted to the identification of most abundant microbiota associated with health or disease. Their abundance may reflect their capacity to exploit their niche, however, metabolic functions exerted… read more here.
Keywords: abundance; low abundant; scoping review; human microbiome ... See more keywords
Isolation of Low-Abundant Bacteroidales in the Human Intestine and the Analysis of Their Differential Utilization Based on Plant-Derived Polysaccharides
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2018.01319
Abstract: Bacteroidales are the most abundant Gram-negative bacteria flourished in the human intestine with great underlying benefits to be discovered and developed as the next-generation probiotics. However, the traditional isolation method limits the mining of low-abundant… read more here.
Keywords: isolation low; abundant bacteroidales; low abundant; human intestine ... See more keywords
Affinity Capture Enrichment versus Affinity Depletion: A Comparison of Strategies for Increasing Coverage of Low-Abundant Human Plasma Proteins
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21165903
Abstract: In the present study, we evaluated four small molecule affinity-based probes based on agarose-immobilized benzamidine (ABA), O-Phospho-L-Tyrosine (pTYR), 8-Amino-hexyl-cAMP (cAMP), or 8-Amino-hexyl-ATP (ATP) for their ability to remove high-abundant proteins such as serum albumin from… read more here.
Keywords: plasma; affinity; low abundant; abundant plasma ... See more keywords