Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9216-4_18
Abstract: We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease… read more here.
Keywords: low copy; copy dsrna; detection low;
How the Investigator Casework GO! Kit provides sensitive, fast and robust direct amplification of low copy number samples
Sign Up to like & getrecommendations! Published in 2019 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2019.10.116
Abstract: Abstract Casework sample processing typically involves DNA extraction, quantification and STR profiling. However, silica-based DNA purification bares the risk of losing crucial sample material especially with low copy number DNA from evidence touch skin samples.… read more here.
Keywords: direct amplification; copy number; low copy; investigator casework ... See more keywords
Development and phylogenetic utilities of a new set of single-/low-copy nuclear genes in Senecioneae (Asteraceae), with new insights into the tribal position and the relationships within subtribe Tussilagininae.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular phylogenetics and evolution"
DOI: 10.1016/j.ympev.2021.107202
Abstract: The tribe Senecioneae is one of the largest tribes in Asteraceae, with a nearly cosmopolitan distribution. Despite great efforts devoted to elucidate the evolution of Senecioneae, many questions still remain concerning the systematics of this… read more here.
Keywords: genes senecioneae; single low; low copy; phylogenetic utilities ... See more keywords
Quantification of Low Copy Number Protein in Single Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b02989
Abstract: We have developed an ultra-sensitive and highly selective method to quantify low copy number intracellular proteins in a single cell using low cost laser induced fluorescence (LIF) detector and BV605 fluorescent probe. Active caspase3 proteins… read more here.
Keywords: copy number; active caspase3; low copy; copy ... See more keywords
Modular Low-Copy-Number Plasmid Vectors for Rhodobacterales with Extended Host Range in Alphaproteobacteria
Sign Up to like & getrecommendations! Published in 2024 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.4c00062
Abstract: Members of the alphaproteobacterial order Rhodobacterales are metabolically diverse and highly abundant in the ocean. They are becoming increasingly interesting for marine biotechnology, due to their ecological adaptability, wealth of versatile low-copy-number plasmids, and their… read more here.
Keywords: low copy; copy number; host; plasmid vectors ... See more keywords
Full Dynamic Range Quantification using Loop-mediated Amplification (LAMP) by Combining Analysis of Amplification Timing and Variance between Replicates at Low Copy Number
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-57473-1
Abstract: Quantification of nucleic acid targets at low copy number is problematic with the limit of detection at 95 percent confidence predicted to be 3 molecules or higher for quantitative PCR. Conversely the accuracy of digital… read more here.
Keywords: copy number; range; low copy; quantification ... See more keywords
Evaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-90959-0
Abstract: Recently, in food safety and various other fields, qualitative and quantitative gene analysis using real-time polymerase chain reaction (PCR) method has become increasingly popular. The limit of detection (LOD) and quantifiable range for these measurements… read more here.
Keywords: copy number; real time; uncertainty; low copy ... See more keywords
Erratum. Metabolomic Profile of Low–Copy Number Carriers at the Salivary α-Amylase Gene Suggests a Metabolic Shift Toward Lipid-Based Energy Production. Diabetes 2016;65:3362–3368
Sign Up to like & getrecommendations! Published in 2017 at "Diabetes"
DOI: 10.2337/db17-er04a
Abstract: In the article listed above, reference 1 was erroneously cited as “Villa R, Mandel AL, Jones BD, La Clair JJ, Burkart MD. … read more here.
Keywords: metabolomic profile; copy number; low copy; profile low ... See more keywords
The 22q11.2 Low Copy Repeats
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13112101
Abstract: LCR22s are among the most complex loci in the human genome and are susceptible to nonallelic homologous recombination. This can lead to a variety of genomic disorders, including deletions, duplications, and translocations, of which the… read more here.
Keywords: 22q11 low; low copy; lcr22s; copy repeats ... See more keywords
A pipeline for assembling low copy nuclear markers from plant genome skimming data for phylogenetic use
Sign Up to like & getrecommendations! Published in 2022 at "PeerJ"
DOI: 10.7717/peerj.14525
Abstract: Background Genome skimming is a popular method in plant phylogenomics that do not include a biased enrichment step, relying on random shallow sequencing of total genomic DNA. From these data the plastome is usually readily… read more here.
Keywords: copy nuclear; genome skimming; low copy; pipeline ... See more keywords