Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9216-4_18
Abstract: We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease… read more here.
Keywords: low copy; copy dsrna; detection low;
How the Investigator Casework GO! Kit provides sensitive, fast and robust direct amplification of low copy number samples
Sign Up to like & getrecommendations! Published in 2019 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2019.10.116
Abstract: Abstract Casework sample processing typically involves DNA extraction, quantification and STR profiling. However, silica-based DNA purification bares the risk of losing crucial sample material especially with low copy number DNA from evidence touch skin samples.… read more here.
Keywords: direct amplification; copy number; low copy; investigator casework ... See more keywords
Development and phylogenetic utilities of a new set of single-/low-copy nuclear genes in Senecioneae (Asteraceae), with new insights into the tribal position and the relationships within subtribe Tussilagininae.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular phylogenetics and evolution"
DOI: 10.1016/j.ympev.2021.107202
Abstract: The tribe Senecioneae is one of the largest tribes in Asteraceae, with a nearly cosmopolitan distribution. Despite great efforts devoted to elucidate the evolution of Senecioneae, many questions still remain concerning the systematics of this… read more here.
Keywords: genes senecioneae; single low; low copy; phylogenetic utilities ... See more keywords
Quantification of Low Copy Number Protein in Single Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b02989
Abstract: We have developed an ultra-sensitive and highly selective method to quantify low copy number intracellular proteins in a single cell using low cost laser induced fluorescence (LIF) detector and BV605 fluorescent probe. Active caspase3 proteins… read more here.
Keywords: copy number; active caspase3; low copy; copy ... See more keywords
Full Dynamic Range Quantification using Loop-mediated Amplification (LAMP) by Combining Analysis of Amplification Timing and Variance between Replicates at Low Copy Number
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-57473-1
Abstract: Quantification of nucleic acid targets at low copy number is problematic with the limit of detection at 95 percent confidence predicted to be 3 molecules or higher for quantitative PCR. Conversely the accuracy of digital… read more here.
Keywords: copy number; range; low copy; quantification ... See more keywords
Evaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-90959-0
Abstract: Recently, in food safety and various other fields, qualitative and quantitative gene analysis using real-time polymerase chain reaction (PCR) method has become increasingly popular. The limit of detection (LOD) and quantifiable range for these measurements… read more here.
Keywords: copy number; real time; uncertainty; low copy ... See more keywords
Erratum. Metabolomic Profile of Low–Copy Number Carriers at the Salivary α-Amylase Gene Suggests a Metabolic Shift Toward Lipid-Based Energy Production. Diabetes 2016;65:3362–3368
Sign Up to like & getrecommendations! Published in 2017 at "Diabetes"
DOI: 10.2337/db17-er04a
Abstract: In the article listed above, reference 1 was erroneously cited as “Villa R, Mandel AL, Jones BD, La Clair JJ, Burkart MD. … read more here.
Keywords: metabolomic profile; copy number; low copy; profile low ... See more keywords
The 22q11.2 Low Copy Repeats
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13112101
Abstract: LCR22s are among the most complex loci in the human genome and are susceptible to nonallelic homologous recombination. This can lead to a variety of genomic disorders, including deletions, duplications, and translocations, of which the… read more here.
Keywords: 22q11 low; low copy; lcr22s; copy repeats ... See more keywords
A pipeline for assembling low copy nuclear markers from plant genome skimming data for phylogenetic use
Sign Up to like & getrecommendations! Published in 2022 at "PeerJ"
DOI: 10.7717/peerj.14525
Abstract: Background Genome skimming is a popular method in plant phylogenomics that do not include a biased enrichment step, relying on random shallow sequencing of total genomic DNA. From these data the plastome is usually readily… read more here.
Keywords: copy nuclear; genome skimming; low copy; pipeline ... See more keywords