Continuous low pH viral inactivation: Operation and scaling strategy informs viral clearance study
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28117
Abstract: A continuous viral inactivation (CVI) tubular reactor was designed for low pH viral inactivation within a continuous downstream system across multiple scales of operation. The reactors were designed to provide a minimum residence time of… read more here.
Keywords: operation; inactivation operation; low viral; viral inactivation ... See more keywords
A Study of Analytical and Clinical Sensitivity of Aptima SARS-CoV-2 Assay (Hologic) and Proposals of Complementary Tests for SARS-CoV-2 Detection in Low Viral Load Specimens
Sign Up to like & getrecommendations! Published in 2021 at "Current Microbiology"
DOI: 10.1007/s00284-021-02730-3
Abstract: Early and accurate detection of SARS-CoV-2 is important for diagnosis and transmission control. The use of high-throughput and automated testing allows laboratories to better deliver diagnostic testing given manpower and resource limitations. We validated the… read more here.
Keywords: sars cov; detection; low viral; aptima sars ... See more keywords
Extremely low viral reservoir in treated chronically HIV-1-infected individuals
Sign Up to like & getrecommendations! Published in 2020 at "EBioMedicine"
DOI: 10.1016/j.ebiom.2020.102830
Abstract: Background Small viral reservoirs are found predominantly in HIV-1 controllers and individuals treated during acute/early HIV-1 infection. However, other HIV+ individuals could naturally also harbour low viral reservoirs. Methods We screened 451 HIV-1-infected treated-individuals with… read more here.
Keywords: hiv dna; viral reservoir; hiv; low viral ... See more keywords
Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2021.114185
Abstract: Objective Viral nucleic acid detection by real-time reverse transcription polymerase chain reaction (qPCR) is the current standard method for diagnosis of SARS-CoV-2 infection. However, due to low viral load in some COVID-19 patients, false negative… read more here.
Keywords: covid patients; detection; low viral; viral load ... See more keywords
Integrating nested PCR with high-throughput sequencing to characterize mutations of HBV genome in low viral load samples
Sign Up to like & getrecommendations! Published in 2017 at "Medicine"
DOI: 10.1097/md.0000000000007588
Abstract: Abstract Due to the low viral load of hepatitis B virus (HBV) in plasma samples, conventional techniques have limitations to the detection of antiviral resistance mutations. To solve the problem, we developed a fast, highly… read more here.
Keywords: low viral; load samples; viral load; load ... See more keywords
Low Viral Diversity Limits the Effectiveness of Sequence-Based Transmission Inference for SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2023 at "mSphere"
DOI: 10.1128/msphere.00544-22
Abstract: Tracking the spread of infection amongst individuals within and between communities has been a major challenge during viral outbreaks. With the unprecedented scale of viral sequence data collection during the severe acute respiratory syndrome coronavirus… read more here.
Keywords: sequence based; sequence; transmission; sars cov ... See more keywords
Transcriptional reprogramming of infiltrating neutrophils drives lung pathology in severe COVID-19 despite low viral load
Sign Up to like & getrecommendations! Published in 2022 at "Blood Advances"
DOI: 10.1182/bloodadvances.2022008834
Abstract: Troubling disparities in COVID-19-associated mortality emerged early, with nearly 70% of deaths confined to Black/African American (AA) patients in some areas. Nevertheless, targeted studies within this vulnerable population are scant. Here, we applied multi-omics single-cell… read more here.
Keywords: reprogramming infiltrating; despite low; viral load; pathology ... See more keywords
Evaluation of the cobas® GT hepatitis C virus genotyping assay in G1-6 viruses including low viral loads and LiPA failures
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0194396
Abstract: Direct-acting antiviral (DAA) drug performances depend on the viral genotype. So international recommendations give typing of the virus a prerequisite for treatment choice and patient management. Commercially available HCV genotyping kits are scarce and this… read more here.
Keywords: lipa failures; genotyping assay; low viral; genotype ... See more keywords
Accurate Detection of SARS-CoV-2 by Next-Generation Sequencing in Low Viral Load Specimens
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24043478
Abstract: As new SARS-CoV-2 variants emerge, there is an urgent need to increase the efficiency and availability of viral genome sequencing, notably to detect the lineage in samples with a low viral load. SARS-CoV-2 genome next-generation… read more here.
Keywords: viral load; next generation; low viral; sars cov ... See more keywords
Analytical Sensitivity of Six SARS-CoV-2 Rapid Antigen Tests for Omicron versus Delta Variant
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14040654
Abstract: Rapid antigen detection (RAD) tests are commonly used for the diagnosis of SARS-CoV-2 infections. However, with the continuous emergence of new variants of concern (VOC), presenting various mutations potentially affecting the nucleocapsid protein, the analytical… read more here.
Keywords: rad tests; rapid antigen; low viral; poor performances ... See more keywords