Establishment of a markerless multiple‐gene deletion method based on Cre/loxP mutant system for Bacillus pumilus
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201700370
Abstract: In this study, we established a Cre/loxP mutant recombination system (Cre/lox71‐66 system) for markerless gene deletion to facilitate our follow‐up rational genetic engineering to the strain Bacillus pumilus W3. This modified method uses two mutant… read more here.
Keywords: system; loxp; cre; method ... See more keywords
Evaluation of a library of loxP variants with a wide range of recombination efficiencies by Cre
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0276657
Abstract: Sparse labeling of individual cells is an important approach in neuroscience and many other fields of research. Various methods have been developed to sparsely label only a small population of cells; however, there is no… read more here.
Keywords: loxp variants; efficiencies cre; library loxp; evaluation library ... See more keywords