Microliter Scale Synthesis of Luciferase‐Encapsulated Polymersomes as Artificial Organelles for Optogenetic Modulation of Cardiomyocyte Beating
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202200239
Abstract: Constructing artificial systems that effectively replace or supplement natural biological machinery within cells is one of the fundamental challenges underpinning bioengineering. At the sub‐cellular scale, artificial organelles (AOs) have significant potential as long‐acting biomedical implants,… read more here.
Keywords: microliter scale; modulation; artificial organelles; synthesis ... See more keywords
Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: PicALuc2.0.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202200560
Abstract: Luciferases are widely used as reporter proteins in diverse fields from basic biology to medical and environmental researches. Development of luciferase applications for reporter proteins requires small size without target inhibition, appropriate genomic insertion for… read more here.
Keywords: luciferase adding; minimal luciferase; improving bioluminescence; bioluminescence minimal ... See more keywords
One-Step Generation of Seamless Luciferase Gene Knockin Using CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9080-1_5
Abstract: Human pluripotent stem cells (hPSCs) offer powerful platforms for studying mechanisms of human diseases and for evaluating potential treatments. Genome editing, particularly the CRISPR/Cas9-based method, is highly effective for generating cell and animal models to… read more here.
Keywords: human pluripotent; pluripotent stem; luciferase; stem ... See more keywords
Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9597-4_8
Abstract: The luciferase immunoprecipitation system (LIPS) assay is a liquid-phase immunoassay that quantitates antigen-specific serum antibodies by measuring luminescence emitted by the reporter enzyme Renilla luciferase (Ruc) fused to an antigen of interest. The LIPS assay… read more here.
Keywords: lips assay; human norovirus; system lips; immunoprecipitation system ... See more keywords
Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-2608-2
Abstract: ObjectivesTo establish a HEK293 cell line with a luciferase knock-in reporter controlled by the endogenous SREBP1 promoter for investigating transcriptional regulation of the SREBP1 gene.ResultsPCR confirmed the site-specific integration of a single copy of the… read more here.
Keywords: srebp1 gene; srebp1; cell line; luciferase ... See more keywords
Early-Phase Luciferase Signals of Islet Grafts Predicts Successful Subcutaneous Site Transplantation in Rats
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Imaging and Biology"
DOI: 10.1007/s11307-020-01560-2
Abstract: The transplantation of pancreatic islets is a promising cell replacement therapy for type 1 diabetes. Subcutaneous islet transplantation is currently under investigation as a means to circumvent problems associated with standard intra-hepatic islet transplantation. As… read more here.
Keywords: phase; subcutaneous islet; islet; bioluminescence ... See more keywords
Etmopterus lantern sharks use coelenterazine as the substrate for their luciferin-luciferase bioluminescence system.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.09.007
Abstract: The lantern shark genus Etmopterus contains approximately 40 species of deep-sea bioluminescent cartilaginous fishes. They emit blue light mainly from the ventral body surface. The biological functions of this bioluminescence have been discussed based on… read more here.
Keywords: lantern sharks; substrate luciferin; bioluminescence; coelenterazine substrate ... See more keywords
A novel dual luciferase based high throughput assay to monitor autophagy in real time in yeast S. cerevisiae
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2017.07.008
Abstract: Background Macroautophagy is a cellular response to starvation wherein superfluous and damaged cytoplasmic constituents are degraded to provide energy for survival and to maintain cellular homeostasis. Dysfunctional autophagy is attributed to disease progression in several… read more here.
Keywords: luciferase based; time; luciferase; dual luciferase ... See more keywords
Increase of segmental mobility through insertion of a flexible linker in split point of firefly luciferase.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2016.03.055
Abstract: The crystal structure of Photinus pyralis luciferase shows a unique molecular architecture consisting of a large N-terminal domain and a small C-terminal domain which is separated by a wide cleft. Protein engineering methods attempts to… read more here.
Keywords: split point; flexible linker; linker; luciferase ... See more keywords
Kinetics, structure, and dynamics of Renilla luciferase solvated in binary mixtures of glycerol and water and the mechanism by which glycerol obstructs the enzyme emitter site.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.05.160
Abstract: Renilla Luciferase is a bioluminescent enzyme which is broadly implemented as protein reporter in biology-related researches. In this study, new evidences on the kinetics, structure, and dynamics of Renilla luciferase solvated in binary mixtures of… read more here.
Keywords: kinetics structure; structure dynamics; luciferase solvated; luciferase ... See more keywords
Generation and characterization of luciferase-secreting, single-round infectious DENV-2 reporter for functional antibody assays.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2021.114119
Abstract: Flavivirus reporters provide a robust tool for viral pathogenesis studies, anti- viral drug screening, disease diagnosis and functional antibody assays. In this study, we generated a luciferase-secreting, single-round reporter virus by replacing the capsid coding… read more here.
Keywords: reporter; reporter virus; luciferase; functional antibody ... See more keywords