Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1221-7_17
Abstract: An understanding of the kinetic contributions to G protein-coupled receptor pharmacology and signaling is increasingly important in compound profiling. Nonequilibrium conditions are commonly present in vivo, for example, as the drug competes with dynamic changes… read more here.
Keywords: complementation approaches; approaches measure; gpcr signaling; pharmacology ... See more keywords
Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation.
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DOI: 10.1007/978-1-0716-1258-3_7
Abstract: G Protein-coupled receptors (GPCRs) transduce signals elicited by bioactive chemical agents (ligands), such as hormones, neurotransmitters, or cytokines, across the cellular membrane. Upon ligand binding, the receptor undergoes structural rearrangements, which cause the activation of… read more here.
Keywords: split luciferase; live cells; activation signaling; luciferase complementation ... See more keywords
Near-Infrared Luciferase Complementation Assay with Enhanced Bioluminescence for Studying Protein-Protein Interactions and Drug Evaluation Under Physiological Conditions.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01238
Abstract: Identification of protein-protein interactions (PPIs) that occur in various cellular processes helps to reveal their potential molecular mechanisms, and there is still an urgent need to develop the assays to explore PPIs in living subjects.… read more here.
Keywords: protein protein; near infrared; luciferase complementation; protein interactions ... See more keywords
A Split Luciferase Complementation Assay for the Quantification of β-Arrestin2 Recruitment to Dopamine D2-Like Receptors
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21176103
Abstract: Investigations on functional selectivity of GPCR ligands have become increasingly important to identify compounds with a potentially more beneficial side effect profile. In order to discriminate between individual signaling pathways, the determination of β-arrestin2 recruitment,… read more here.
Keywords: split luciferase; complementation assay; luciferase complementation; arrestin2 recruitment ... See more keywords