Assembly of Metabolons in Yeast Using Cas6-Mediated RNA Scaffolding.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00650
Abstract: Cells often localize pathway enzymes in close proximity to reduce substrate loss via diffusion and to ensure that carbon flux is directed toward the desired product. To emulate this strategy for the biosynthesis of heterologous… read more here.
Keywords: rna; cas6; luminescence signal; mediated rna ... See more keywords
Inhibitor bias in luciferase-based luminescence assays
Sign Up to like & getrecommendations! Published in 2020 at "Future Science OA"
DOI: 10.2144/fsoa-2020-0081
Abstract: The firefly luciferase (FLuc)-based bioluminescent reaction [1] provides the basis for one of the most popular detection systems in high-throughput screening (HTS) [2], especially for cell-based (reporter gene) assays [2,3]. Bioluminescence results from the activity… read more here.
Keywords: reporter; luminescence signal; inhibition; fluc inhibition ... See more keywords