Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7896-0_18
Abstract: Pulmonary dendritic cells (DCs) are potent antigen-presenting cells that can activate both naïve and memory/effector T cells. However, very little is known of how movements and localization of DCs contribute to these events. To study… read more here.
Keywords: cut lung; precision cut; lung slices; localization ... See more keywords
Precision cut lung slices: an ex vivo model for assessing the impact of immunomodulatory therapeutics on lung immune responses.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of toxicology"
DOI: 10.1007/s00204-021-03096-y
Abstract: Chronic inflammatory diseases of the respiratory tract, such as chronic obstructive pulmonary disease (COPD) and asthma, are severe lung diseases that require effective treatments. In search for new medicines for these diseases, there is an… read more here.
Keywords: cut lung; precision cut; vivo model; lung slices ... See more keywords
siRNA‐mediated protein knockdown in precision‐cut lung slices
Sign Up to like & getrecommendations! Published in 2018 at "European Journal of Pharmaceutics and Biopharmaceutics"
DOI: 10.1016/j.ejpb.2018.11.005
Abstract: &NA; Small interfering RNA (siRNA) can induce RNA interference, which leads to the knockdown of messenger RNA (mRNA) and protein. As a result, siRNA is often used in vitro and in vivo to unravel the… read more here.
Keywords: protein knockdown; sirna; precision cut; lung slices ... See more keywords
Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for “on-demand” use and long-term cultures
Sign Up to like & getrecommendations! Published in 2023 at "Toxicological Sciences"
DOI: 10.1093/toxsci/kfac136
Abstract: Abstract Human precision-cut lung slices (hPCLS), considered a highly relevant ex vivo model of the lung, offer native architecture and cells of the lung tissue including respiratory parenchyma, small airways, and immune competent cells. However,… read more here.
Keywords: cut lung; lung slices; lung; human precision ... See more keywords
Precision-cut lung slices: a powerful ex vivo model to investigate respiratory infectious diseases.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular microbiology"
DOI: 10.1111/mmi.14817
Abstract: Respiratory infections are leading causes of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid or surrogate animal models. Although these provide important insights, they have limitations.… read more here.
Keywords: cut lung; precision cut; lung slices; respiratory ... See more keywords
Multiscale stiffness of human emphysematous precision cut lung slices
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.adf2535
Abstract: Emphysema is a debilitating disease that remodels the lung leading to reduced tissue stiffness. Thus, understanding emphysema progression requires assessing lung stiffness at both the tissue and alveolar scales. Here, we introduce an approach to… read more here.
Keywords: precision cut; cut lung; stiffness; lung ... See more keywords
Contribution of TRPV4 Channels in the Intrinsic Calcium Signaling Profiles of the Mouse Lung Microvascular Endothelium
Sign Up to like & getrecommendations! Published in 2023 at "Physiology"
DOI: 10.1152/physiol.2023.38.s1.5733755
Abstract: Endothelial calcium signaling in lung microvasculature is known to play an important role in maintaining lung endothelial barrier integrity. We previously characterized an intrinsic dynamic endothelial Ca2+ signaling pattern of the mouse lung microcirculation. Recently,… read more here.
Keywords: calcium; trpv4 channels; trpv4; physiology ... See more keywords
A tunable physiomimetic stretch system evaluated with precision cut lung slices and recellularized human lung scaffolds
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.995460
Abstract: Breathing exposes lung cells to continual mechanical stimuli, which is part of the microenvironmental signals directing cellular functions together with the extracellular matrix (ECM). Therefore, developing systems that incorporate both stimuli is urgent to fully… read more here.
Keywords: methodology; stretch; precision cut; lung ... See more keywords
Perspectives on precision cut lung slices—powerful tools for investigation of mechanisms and therapeutic targets in lung diseases
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2023.1162889
Abstract: Precision cut lung slices (PCLS) have emerged as powerful experimental tools for respiratory research. Pioneering studies using mouse PCLS to visualize intrapulmonary airway contractility have been extended to pulmonary arteries and for assessment of novel… read more here.
Keywords: mechanisms therapeutic; precision cut; cut lung; lung ... See more keywords
Application of Precision-Cut Lung Slices as an In Vitro Model for Research of Inflammatory Respiratory Diseases
Sign Up to like & getrecommendations! Published in 2022 at "Bioengineering"
DOI: 10.3390/bioengineering9120767
Abstract: The leading cause of many respiratory diseases is an ongoing and progressive inflammatory response. Traditionally, inflammatory lung diseases were studied primarily through animal models, cell cultures, and organoids. These technologies have certain limitations, despite their… read more here.
Keywords: precision cut; lung; model; lung slices ... See more keywords