Articles with "lysate" as a keyword



Blastomyces dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared from Human Isolates from a Blastomycosis Outbreak

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Published in 2019 at "Mycopathologia"

DOI: 10.1007/s11046-019-00385-0

Abstract: Blastomycosis is a systemic fungal disease of humans and other animals produced by the thermally dimorphic fungal organism, Blastomyces dermatitidis. Recent studies have focused on the utilization of antibody and antigen detection in the development… read more here.

Keywords: detection; antibody; lysate; antigen ... See more keywords

Universal efavirenz determination in transport study, rat placenta perfusion and placenta lysate by HPLC‐UV

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Published in 2017 at "Journal of Pharmaceutical and Biomedical Analysis"

DOI: 10.1016/j.jpba.2017.01.012

Abstract: &NA; Efavirenz is an antiretroviral drug used in the treatment of HIV‐positive patients. A simple, fast and sensitive high‐performance liquid chromatography (HPLC) method was developed in order to determine efavirenz in three types of samples… read more here.

Keywords: hplc; perfusion; determination; transport ... See more keywords

Diffusion NMR and Rheology of a Model Polymer in Bacterial Cell Lysate Crowders.

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Published in 2023 at "Biomacromolecules"

DOI: 10.1021/acs.biomac.2c01534

Abstract: The intracellular milieu is crowded and heterogeneous, and this can have profound consequences for biomolecule motions and biochemical kinetics. Macromolecular crowding has been traditionally studied in artificial crowders like Ficoll and dextran or globular proteins… read more here.

Keywords: rheology; bacterial cell; cell lysate; lysate ... See more keywords

Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment.

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Published in 2017 at "Journal of the American Chemical Society"

DOI: 10.1021/jacs.6b11058

Abstract: The intramolecular electric field (e-field) generated by protein GB3 side-chain charges K/E10, K/E19, and D/K40 was measured in the absence or presence of macromolecular crowding. The e-field responds differently to different crowding agents-dextran, Ficoll, BSA,… read more here.

Keywords: field generated; field; side chain; lysate ... See more keywords

P19 Defining the mechanism of skin barrier restoration by Lactobacillus rhamnosus

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Published in 2024 at "British Journal of Dermatology"

DOI: 10.1093/bjd/ljae105.041

Abstract: We aim to investigate the lysate of Lactobacillus rhamnosus GG (LGG), a bacterium previously established as a gut probiotic, and to examine its postulated ability to enhance and restore human skin barrier function. Compromised skin… read more here.

Keywords: skin barrier; lactobacillus rhamnosus; lgg lysate; tight junction ... See more keywords

Autologous Dendritic Cells Pulsed with Allogeneic Tumor Cell Lysate in Mesothelioma: From Mouse to Human

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Published in 2017 at "Clinical Cancer Research"

DOI: 10.1158/1078-0432.ccr-17-2522

Abstract: Purpose: Mesothelioma has been regarded as a nonimmunogenic tumor, which is also shown by the low response rates to treatments targeting the PD-1/PD-L1 axis. Previously, we demonstrated that autologous tumor lysate–pulsed dendritic cell (DC) immunotherapy… read more here.

Keywords: mesothelioma; tumor lysate; lysate; allogeneic tumor ... See more keywords

Characterization of vitamin D3 biotransformation by the cell lysate of Actinomyces hyovaginalis CCASU-A11-2

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Published in 2024 at "AMB Express"

DOI: 10.1186/s13568-024-01694-4

Abstract: A former work conducted in our Lab, lead to in a effective scale up of vitamin D3 bioconversion into calcitriol by Actinomyces (A.) hyovaginalis isolate CCASU-A11-2 in Lab fermenter (14 L) resulting in 32.8 µg/100 mL of… read more here.

Keywords: cell free; calcitriol; ccasu a11; cell ... See more keywords

Immunization of mice with soluble lysate of interferon gamma expressing Plasmodium berghei ANKA induces high IFN-γ production

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Published in 2017 at "Tropical Diseases, Travel Medicine and Vaccines"

DOI: 10.1186/s40794-017-0053-1

Abstract: BackgroundEfforts in search of lasting malaria vaccine have led to the development of transgenic rodent malaria parasites. As a result, wild type Plasmodium berghei ANKA (WTPbA) has recently been transformed to express mouse interferon gamma… read more here.

Keywords: immunization; production; group; lysate ... See more keywords

Development and comparison of immunologic assays to detect primary RSV infections in infants

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Published in 2024 at "Frontiers in Immunology"

DOI: 10.3389/fimmu.2023.1332772

Abstract: Effective respiratory syncytial virus (RSV) vaccines have been developed and licensed for elderly adults and pregnant women but not yet for infants and young children. The RSV immune state of the young child, i.e., previously… read more here.

Keywords: assays detect; antibody assays; antibody; rsv ... See more keywords

Lysate of Parabacteroides distasonis prevents severe forms of experimental autoimmune encephalomyelitis by modulating the priming of T cell response

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Published in 2024 at "Frontiers in Immunology"

DOI: 10.3389/fimmu.2024.1475126

Abstract: The gut microbiota influences the reactivity of the immune system, and Parabacteroides distasonis has emerged as an anti-inflammatory commensal. Here, we investigated whether its lysate could prevent severe forms of neuroinflammation in experimental autoimmune encephalomyelitis… read more here.

Keywords: response; severe forms; cell; lysate ... See more keywords

Multiple Proteins of Lacticaseibacillus rhamnosus GG Are Involved in the Protection of Keratinocytes From the Toxic Effects of Staphylococcus aureus

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Published in 2022 at "Frontiers in Microbiology"

DOI: 10.3389/fmicb.2022.875542

Abstract: We have previously shown that lysates of Lacticaseibacillus rhamnosus GG confer protection to human keratinocytes against Staphylococcus aureus. L. rhamnosus GG inhibits the growth of S. aureus as well as competitively excludes and displaces the… read more here.

Keywords: adhesion; protection; anti adhesive; staphylococcus aureus ... See more keywords