Kinetic Studies of the Effect of pH on the Trypsin-Catalyzed Hydrolysis of N-α-benzyloxycarbonyl-l-lysine-p-nitroanilide: Mechanism of Trypsin Catalysis
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DOI: 10.1021/acsomega.9b03750
Abstract: The pH dependence of the trypsin-catalyzed hydrolysis of N-α-benzyloxycarbonyl-l-lysine p-nitroanilide has been studied at 25 °C. kcat/KM was maximal at alkaline pH values but decreased with decreasing pH. kcat/KM was dependent on free enzyme pKa… read more here.
Keywords: hydrolysis benzyloxycarbonyl; benzyloxycarbonyl lysine; lysine nitroanilide; trypsin ... See more keywords