New horizons in developing cell lysis methods: A review
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28198
Abstract: Cell lysis is an essential step in many studies related to biology and medicine. Based on the scale and medium that cell lysis is carried out, there are three main types of the cell lysis:… read more here.
Keywords: cell lysis; new horizons; lysis; lysis methods ... See more keywords
Design of experiments‐based high‐throughput strategy for development and optimization of efficient cell disruption protocols
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600030
Abstract: Efficient and reproducible cell lysis is a crucial step during downstream processing of intracellular products. The composition of an optimal lysis buffer should be chosen depending on the organism, its growth status, the applied detection… read more here.
Keywords: design experiments; lysis buffer; lysis; high throughput ... See more keywords
Lipid‐based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.12200
Abstract: Abstract Flow cytometry (FCM) is a popular method used in characterisation of extracellular vesicles (EVs). Circulating EVs are often identified by FCM by exploiting the lipid nature of EVs by staining with Annexin V (Anx5)… read more here.
Keywords: extracellular vesicles; lysis; flow cytometry; evs ... See more keywords
Merkel cell carcinoma and cellular cytotoxicity: sensitivity to cellular lysis and screening for potential target antigens suitable for antibody-dependent cellular cytotoxicity
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Immunology, Immunotherapy"
DOI: 10.1007/s00262-018-2176-2
Abstract: The recent success of checkpoint inhibitors in the treatment of Merkel cell carcinoma (MCC) confirms that MCC tumors can be immunogenic. However, no treatment directly targeting the tumor is available for use in combination with… read more here.
Keywords: sensitivity cellular; cell; lysis; cellular cytotoxicity ... See more keywords
Lyse-it™: a Single Step, Single Platform, Transformational Lysing Technology
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Fluorescence"
DOI: 10.1007/s10895-017-2043-0
Abstract: There is a long-standing need for rapid and sensitive detection platforms, including point-of-care tests, for a variety of viruses and bacteria. While many technologies such as PCR and others, have the potential to provide results… read more here.
Keywords: single platform; platform; lysis; technology ... See more keywords
Algal Lysis by Sagittula stellata for the Production of Intracellular Valuables
Sign Up to like & getrecommendations! Published in 2021 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03502-2
Abstract: The purpose of this study was to examine the efficacy of the algicidal bacterium Sagittula stellata on the cell lysis of Nannochloropsis oceanica, a microalga found in the marine environment, in order to extract intracellular… read more here.
Keywords: stellata; sagittula stellata; lysis; cell ... See more keywords
A Novel Direct PCR Lysis Buffer Can Improve PCR from Meat Matrices
Sign Up to like & getrecommendations! Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1342-7
Abstract: Molecular technologies based on PCR have been widely used in many biological analysis fields, especially for genetic analysis and DNA barcoding. In this study, a rapid DNA lysis liquid was formulated without any purification step… read more here.
Keywords: direct pcr; dna; pcr; meat ... See more keywords
Development of a simple and efficient method of harvesting and lysing adherent mammalian cells for chemical isotope labeling LC-MS-based cellular metabolomics.
Sign Up to like & getrecommendations! Published in 2018 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.11.054
Abstract: Chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) has a potential to become a very powerful platform for comprehensive and quantitative analysis of metabolites in cellular metabolomics. While CIL LC-MS with rationally designed reagents… read more here.
Keywords: harvesting lysing; cellular metabolomics; cell harvest; lysis ... See more keywords
Research progress on bacterial ghosts as novel fishery vaccines
Sign Up to like & getrecommendations! Published in 2022 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2021.737526
Abstract: Abstract Outbreaks of fish bacterial diseases have become quite frequent with the intensification of practices in aquaculture. Vaccination has become an increasingly important prevention strategy against infectious agents in farmed fishes. However, only a few… read more here.
Keywords: research; mediated lysis; bacterial ghosts; lysis ... See more keywords
A luciferase lysis assay reveals in vivo malignant cell sensitization by phosphoantigen prodrugs.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical pharmacology"
DOI: 10.1016/j.bcp.2019.113668
Abstract: Human Vγ9Vδ2 T cells respond to small phosphorus-containing compounds, often called phosphoantigens, which are now known to be intracellular ligands of the immune receptor butyrophilin 3A1 (BTN3A1). In order to compare the efficiency of butyrophilin… read more here.
Keywords: lysis assay; luciferase lysis; phosphoantigen prodrugs; lysis ... See more keywords
A Host-Produced Quorum-Sensing Autoinducer Controls a Phage Lysis-Lysogeny Decision
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2018.10.059
Abstract: Vibrio cholerae uses a quorum-sensing (QS) system composed of the autoinducer 3,5-dimethylpyrazin-2-ol (DPO) and receptor VqmA (VqmAVc), which together repress genes for virulence and biofilm formation. vqmA genes exist in Vibrio and in one vibriophage,… read more here.
Keywords: phage; lysogeny; quorum sensing; lysis ... See more keywords