Mechano‐Lysis in Whole Blood Clots: On How Mechanics Affect Clot Lysis, and How Lysis Affects Clot Mechanics
Sign Up to like & getrecommendations! Published in 2025 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202403389
Abstract: Thromboembolic diseases are a significant cause of mortality and are clinically treated enzymatically with tissue plasminogen activator (tPA). Interestingly, prior studies in fibrin fibers and fibrin gels have demonstrated that thrombolysis may be mechanically sensitive.… read more here.
Keywords: blood clots; whole blood; clot; mechanics ... See more keywords
New horizons in developing cell lysis methods: A review
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28198
Abstract: Cell lysis is an essential step in many studies related to biology and medicine. Based on the scale and medium that cell lysis is carried out, there are three main types of the cell lysis:… read more here.
Keywords: cell lysis; new horizons; lysis; lysis methods ... See more keywords
Design of experiments‐based high‐throughput strategy for development and optimization of efficient cell disruption protocols
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600030
Abstract: Efficient and reproducible cell lysis is a crucial step during downstream processing of intracellular products. The composition of an optimal lysis buffer should be chosen depending on the organism, its growth status, the applied detection… read more here.
Keywords: design experiments; lysis buffer; lysis; high throughput ... See more keywords
Lipid‐based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.12200
Abstract: Abstract Flow cytometry (FCM) is a popular method used in characterisation of extracellular vesicles (EVs). Circulating EVs are often identified by FCM by exploiting the lipid nature of EVs by staining with Annexin V (Anx5)… read more here.
Keywords: extracellular vesicles; lysis; flow cytometry; evs ... See more keywords
Long-term CT follow-up of patients with lumbar spondylolysis reveals low rate of spontaneous bone fusion
Sign Up to like & getrecommendations! Published in 2024 at "Skeletal Radiology"
DOI: 10.1007/s00256-024-04650-2
Abstract: Knowledge about the long-term outcome of patients with lumbar spondylolysis (LS) is limited. This study assessed the frequency of bone fusion in conservatively treated lumbar spondylolysis with photon counting detector computed tomography. Patients with lumbar… read more here.
Keywords: fusion; bone fusion; lysis; lumbar spondylolysis ... See more keywords
Merkel cell carcinoma and cellular cytotoxicity: sensitivity to cellular lysis and screening for potential target antigens suitable for antibody-dependent cellular cytotoxicity
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Immunology, Immunotherapy"
DOI: 10.1007/s00262-018-2176-2
Abstract: The recent success of checkpoint inhibitors in the treatment of Merkel cell carcinoma (MCC) confirms that MCC tumors can be immunogenic. However, no treatment directly targeting the tumor is available for use in combination with… read more here.
Keywords: sensitivity cellular; cell; lysis; cellular cytotoxicity ... See more keywords
Lyse-it™: a Single Step, Single Platform, Transformational Lysing Technology
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Fluorescence"
DOI: 10.1007/s10895-017-2043-0
Abstract: There is a long-standing need for rapid and sensitive detection platforms, including point-of-care tests, for a variety of viruses and bacteria. While many technologies such as PCR and others, have the potential to provide results… read more here.
Keywords: single platform; platform; lysis; technology ... See more keywords
Algal Lysis by Sagittula stellata for the Production of Intracellular Valuables
Sign Up to like & getrecommendations! Published in 2021 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03502-2
Abstract: The purpose of this study was to examine the efficacy of the algicidal bacterium Sagittula stellata on the cell lysis of Nannochloropsis oceanica, a microalga found in the marine environment, in order to extract intracellular… read more here.
Keywords: stellata; sagittula stellata; lysis; cell ... See more keywords
A Novel Direct PCR Lysis Buffer Can Improve PCR from Meat Matrices
Sign Up to like & getrecommendations! Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1342-7
Abstract: Molecular technologies based on PCR have been widely used in many biological analysis fields, especially for genetic analysis and DNA barcoding. In this study, a rapid DNA lysis liquid was formulated without any purification step… read more here.
Keywords: direct pcr; dna; pcr; meat ... See more keywords
Physicochemical cell disruption of Bacillus sp. for recovery of polyhydroxyalkanoates: future bioplastic for sustainability
Sign Up to like & getrecommendations! Published in 2024 at "3 Biotech"
DOI: 10.1007/s13205-024-03913-y
Abstract: Polyhydroxybutyrate (PHB) is known for wide applications, biocompatibility, and degradability; however, it cannot be commercialized due to conventional recovery using solvents. The present study employed mechanical cell-disruption methods, such as Pestle and mortar, sonication, and… read more here.
Keywords: cell disruption; recovery; cell; lysis ... See more keywords
Development of a simple and efficient method of harvesting and lysing adherent mammalian cells for chemical isotope labeling LC-MS-based cellular metabolomics.
Sign Up to like & getrecommendations! Published in 2018 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.11.054
Abstract: Chemical isotope labeling (CIL) liquid chromatography mass spectrometry (LC-MS) has a potential to become a very powerful platform for comprehensive and quantitative analysis of metabolites in cellular metabolomics. While CIL LC-MS with rationally designed reagents… read more here.
Keywords: harvesting lysing; cellular metabolomics; cell harvest; lysis ... See more keywords