Design of experiments‐based high‐throughput strategy for development and optimization of efficient cell disruption protocols
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DOI: 10.1002/elsc.201600030
Abstract: Efficient and reproducible cell lysis is a crucial step during downstream processing of intracellular products. The composition of an optimal lysis buffer should be chosen depending on the organism, its growth status, the applied detection… read more here.
Keywords: design experiments; lysis buffer; lysis; high throughput ... See more keywords
A Novel Direct PCR Lysis Buffer Can Improve PCR from Meat Matrices
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DOI: 10.1007/s12161-018-1342-7
Abstract: Molecular technologies based on PCR have been widely used in many biological analysis fields, especially for genetic analysis and DNA barcoding. In this study, a rapid DNA lysis liquid was formulated without any purification step… read more here.
Keywords: direct pcr; dna; pcr; meat ... See more keywords
Physicochemical cell disruption of Bacillus sp. for recovery of polyhydroxyalkanoates: future bioplastic for sustainability
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DOI: 10.1007/s13205-024-03913-y
Abstract: Polyhydroxybutyrate (PHB) is known for wide applications, biocompatibility, and degradability; however, it cannot be commercialized due to conventional recovery using solvents. The present study employed mechanical cell-disruption methods, such as Pestle and mortar, sonication, and… read more here.
Keywords: cell disruption; recovery; cell; lysis ... See more keywords
A-356 A hand-held microfluidic platform for rapid and sensitive identification of respiratory viruses from nasal swab samples
Sign Up to like & getrecommendations! Published in 2024 at "Clinical Chemistry"
DOI: 10.1093/clinchem/hvae106.350
Abstract: Identifying the infected individual with a fast and accurate test is essential to constrain the spread of viruses and provide the proper treatment. Antigen rapid test (ART) provides quick screening of infection for patients; however,… read more here.
Keywords: detection; hand held; hand; lysis buffer ... See more keywords
Evaluation of blood culture broths with lysis buffer to directly identify specific pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry methods
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DOI: 10.2147/idr.s169197
Abstract: Background Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now widely used to detect pathogens in clinical settings in Japan. Method Here, we report the effects of adding lysis buffer in the MALDI-TOF MS… read more here.
Keywords: lysis buffer; lysis; blood culture;
Limited efficacy of somatic cell lysis buffer to purify laboratory mouse sperm.
Sign Up to like & getrecommendations! Published in 2018 at "Epigenomics"
DOI: 10.2217/epi-2018-0015
Abstract: AIM Epigenetic analyses of sperm require pure samples devoid of diploid cell contamination. We sought to determine the efficacy of somatic cell lysis buffer (SCLB) treatment to purify mouse epididymis sperm samples. MATERIALS & METHODS… read more here.
Keywords: somatic cell; efficacy somatic; cell lysis; sperm ... See more keywords