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Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600030
Abstract: Efficient and reproducible cell lysis is a crucial step during downstream processing of intracellular products. The composition of an optimal lysis buffer should be chosen depending on the organism, its growth status, the applied detection…
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Keywords:
design experiments;
lysis buffer;
lysis;
high throughput ... See more keywords
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Published in 2018 at "Food Analytical Methods"
DOI: 10.1007/s12161-018-1342-7
Abstract: Molecular technologies based on PCR have been widely used in many biological analysis fields, especially for genetic analysis and DNA barcoding. In this study, a rapid DNA lysis liquid was formulated without any purification step…
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Keywords:
direct pcr;
dna;
pcr;
meat ... See more keywords
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Published in 2018 at "Infection and Drug Resistance"
DOI: 10.2147/idr.s169197
Abstract: Background Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now widely used to detect pathogens in clinical settings in Japan. Method Here, we report the effects of adding lysis buffer in the MALDI-TOF MS…
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Keywords:
lysis buffer;
lysis;
blood culture;
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Published in 2018 at "Epigenomics"
DOI: 10.2217/epi-2018-0015
Abstract: AIM Epigenetic analyses of sperm require pure samples devoid of diploid cell contamination. We sought to determine the efficacy of somatic cell lysis buffer (SCLB) treatment to purify mouse epididymis sperm samples. MATERIALS & METHODS…
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Keywords:
somatic cell;
efficacy somatic;
cell lysis;
sperm ... See more keywords