The Melolontha melolontha entomopoxvirus fusolin protein is a chitin‐active lytic polysaccharide monooxygenase that displays extreme stability
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14620
Abstract: Spindles are intracellular crystals of the fusolin protein that enhances the oral virulence of insect poxviruses by disruption of the larval chitinous peritrophic matrix. The enigmatic fusolin protein is classified as a lytic polysaccharide monooxygenase… read more here.
Keywords: lytic polysaccharide; fusolin; fusolin protein; extreme stability ... See more keywords
Reductants fuel lytic polysaccharide monooxygenase activity in a pH‐dependent manner
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14629
Abstract: Polysaccharide‐degrading mono‐copper lytic polysaccharide monooxygenases (LPMOs) are efficient peroxygenases that require electron donors (reductants) to remain in the active Cu(I) form and to generate the H2O2 co‐substrate from molecular oxygen. Here, we show how commonly… read more here.
Keywords: lytic polysaccharide; dependent manner; fuel lytic; reductants fuel ... See more keywords
Unfolding of CBP21, a lytic polysaccharide monooxygenase, without dissociation of its copper ion cofactor.
Sign Up to like & getrecommendations! Published in 2019 at "Biopolymers"
DOI: 10.1002/bip.23339
Abstract: Chitin-binding protein 21 (CBP21) from Serratia marcescens is a lytic polysaccharide monooxygenase that contains a copper ion as a cofactor. We aimed to elucidate the unfolding mechanism of CBP21 and the effects of Cu2+ on… read more here.
Keywords: cu2; polysaccharide monooxygenase; copper ion; lytic polysaccharide ... See more keywords
Enzymatic degradation of algal 1,3-xylan: from synergism of lytic polysaccharide monooxygenases with β-1,3-xylanases to their intelligent immobilization on biomimetic silica nanoparticles
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10624-w
Abstract: Abstract Lytic polysaccharide monooxygenases (LPMOs) with synergistic effect on polysaccharide hydrolase represent a revolution in biotechnology, which may accelerate the conversion of biomass to the second-generation biofuels. Discovering more hydrolases that have synergism with LPMOs… read more here.
Keywords: immobilization; biomass; synergism; polysaccharide monooxygenases ... See more keywords
PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus.
Sign Up to like & getrecommendations! Published in 2020 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-020-10911-6
Abstract: Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large… read more here.
Keywords: psaa9a; pycnoporus; white rot; sanguineus ... See more keywords
A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02760-9
Abstract: Objective The development of an enzymatic assay for the specific quantification of the C1-oxidation product, i.e. gluconic acid of cellulose active lytic polysaccharide monooxygenases (LPMOs). Results In combination with a β-glucosidase, the spectrophotometrical assay can… read more here.
Keywords: oxidation; enzymatic assay; assay; polysaccharide monooxygenases ... See more keywords
Lytic polysaccharide monooxygenases as powerful tools in enzymatically assisted preparation of nano-scaled cellulose from lignocellulose: A review.
Sign Up to like & getrecommendations! Published in 2021 at "Bioresource technology"
DOI: 10.1016/j.biortech.2021.126491
Abstract: Nanocellulose, either in the form of fibers or crystals, constitutes a renewable, biobased, biocompatible material with advantageous mechanical properties that can be isolated from lignocellulosic biomass. Enzyme-assisted isolation of nanocellulose is an attractive, environmentally friendly… read more here.
Keywords: scaled cellulose; lytic polysaccharide; preparation nano; polysaccharide monooxygenases ... See more keywords
Heterologous expression and characterization of a novel lytic polysaccharide monooxygenase from Natrialbaceae archaeon and its application for chitin biodegradation.
Sign Up to like & getrecommendations! Published in 2022 at "Bioresource technology"
DOI: 10.1016/j.biortech.2022.127174
Abstract: Lytic polysaccharide monooxygenases could enhance the enzymatic conversion of recalcitrant polysaccharides by glycoside hydrolases. This study reports the expression and identification of a novel AA10 LPMO from Natrialbaceae archaeon, named NaLPMO10A, as a C1 oxidizer… read more here.
Keywords: expression; chitin; lytic polysaccharide; natrialbaceae archaeon ... See more keywords
Enhanced konjac glucomannan hydrolysis by lytic polysaccharide monooxygenases and generating prebiotic oligosaccharides.
Sign Up to like & getrecommendations! Published in 2021 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2020.117241
Abstract: Konjac glucomannan (KGM) hydrolysate is a potentially valuable prebiotic that could improve gastrointestinal health by modulating the growth of probiotic bacteria and by promoting the production of short-chain fatty acids (SCFAs). In this study, we… read more here.
Keywords: enhanced konjac; konjac glucomannan; polysaccharide; polysaccharide monooxygenases ... See more keywords
A novel expression system for lytic polysaccharide monooxygenases.
Sign Up to like & getrecommendations! Published in 2017 at "Carbohydrate research"
DOI: 10.1016/j.carres.2017.02.003
Abstract: Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive lignocellulose saccharification. The recombinant production of LPMOs… read more here.
Keywords: system; expression system; expression; polysaccharide monooxygenases ... See more keywords
Oxidative cleavage and hydrolytic boosting of cellulose in soybean spent flakes by Trichoderma reesei Cel61A lytic polysaccharide monooxygenase.
Sign Up to like & getrecommendations! Published in 2017 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2016.12.007
Abstract: The auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenase (LPMO) from Trichoderma reesei (EG4; TrCel61A) was investigated for its ability to oxidize the complex polysaccharides from soybean. The substrate specificity of the enzyme was… read more here.
Keywords: polysaccharide monooxygenase; lytic polysaccharide; trichoderma reesei; soy spent ... See more keywords