Design of a microfluidic mixer channel: First steps into creating a fluorescent dye‐based biosensor for mAb aggregate detection
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200332
Abstract: A major challenge in the transition to continuous biomanufacturing is the lack of process analytical technology (PAT) tools which are able to collect real‐time information on the process and elicit a response to facilitate control.… read more here.
Keywords: mab; time; design; detection ... See more keywords
Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26278
Abstract: Antibody interchain disulfide bond reduction during biopharmaceutical manufacturing has received increased attention since it was first reported in 2010. Antibody reduction leads to loss of product and reduced product stability. It is therefore critical to… read more here.
Keywords: reduction; system; antibody; thioredoxin ... See more keywords
Application of a fluorescent dye-based microfluidic sensor for real-time detection of mAb aggregates.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3355
Abstract: The lack of process analytical technologies able to provide real-time information and process control over a biopharmaceutical process has long impaired the transition to continuous biomanufacturing. For the monoclonal antibody (mAb) production, aggregate formation is… read more here.
Keywords: mab; time; dye based; real time ... See more keywords
Purification of Human Monoclonal Antibodies and Their Fragments.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8958-4_7
Abstract: This chapter summarizes the most common chromatographic mAb and mAb fragment purification methods, starting by elucidating the relevant properties of the compounds and introducing the various chromatography modes that are available and useful for this… read more here.
Keywords: chromatography; mab; purity; purification human ... See more keywords
A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen
Sign Up to like & getrecommendations! Published in 2021 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-021-11342-7
Abstract: Abstract To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)–specific monoclonal antibody (mAb), the Escherichia coli–expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction… read more here.
Keywords: antibody; capsid protein; hev; capsid ... See more keywords
Altered binding avidities and improved growth inhibitory effects of novel anti-HER3 mAb against human cancers in the presence of HER1-or HER2-targeted drugs.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.08.091
Abstract: HER1-and HER2-targeted drugs are effective in cancer therapy, especially against lung, breast and colon malignancies; however, resistance of cancer cells to HER1-and HER2-targeted therapies is becoming a serious problem. The avidity/affinity constant (KA) and growth… read more here.
Keywords: presence; her1; her1 her2; her2 ... See more keywords
A novel IgG1 monoclonal antibody against xanthine oxidase alleviates inflammation induced by potassium oxonate in mice.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.01.171
Abstract: Xanthine oxidase (XOD) is a key enzyme that catalyzes xanthine to uric acid. Most of the urate-lowering medicines targeting XOD have a limited effect on alleviating inflammation in spite of significant effects on decreasing serum… read more here.
Keywords: xod; xanthine oxidase; inflammation; potassium oxonate ... See more keywords
Influence of glycan modification on IgG1 biochemical and biophysical properties
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2017.12.061
Abstract: Graphical abstract Figure. No caption available. HighlightsGlycosylation affects both physico‐chemical properties of mAbs and their interactions with the key receptors.Fully deglycosylated, hypergalactosylated and hypersialylated mAb molecules were produced and characterized.Aggregation propensity is not increased for… read more here.
Keywords: igg1; influence glycan; ggr; hypergalactosylated hypersialylated ... See more keywords
Characterization of a variant CD4 molecule in Japanese Black cattle.
Sign Up to like & getrecommendations! Published in 2020 at "Veterinary immunology and immunopathology"
DOI: 10.1016/j.vetimm.2020.110167
Abstract: Monoclonal antibodies (mAbs) that recognize cluster of differentiation (CD) molecules on lymphocytes are useful tools for the study of different lymphocyte subsets in flow cytometry (FCM) analysis. CD4 is a glycoprotein found on the surfaces… read more here.
Keywords: pbmcs; japanese black; characterization variant; mab ... See more keywords
Anti-High Mobility Group Box 1 Antibody Therapy May Prevent Cognitive Dysfunction After Traumatic Brain Injury.
Sign Up to like & getrecommendations! Published in 2019 at "World neurosurgery"
DOI: 10.1016/j.wneu.2018.10.164
Abstract: BACKGROUND High mobility group box 1 (HMGB1) protein plays a key role in triggering inflammatory responses in many diseases. Our previous study showed that HMGB1 is found upstream of secondary damage in traumatic brain injury… read more here.
Keywords: anti hmgb1; hmgb1 mab; brain; injury ... See more keywords
Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c03654
Abstract: Quantitation of therapeutic monoclonal antibodies (mAbs) in human serum could ensure that patients have adequate levels of mAbs for effective treatment. This research describes the use of affinity, glass-fiber membranes in a 96-well-plate format for… read more here.
Keywords: mab; affinity; quantitation; well plates ... See more keywords