High Macromolecular Crowding in Liposomes from Microfluidics
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202201169
Abstract: The intracellular environment is crowded with macromolecules that influence biochemical equilibria and biomacromolecule diffusion. The incorporation of such crowding in synthetic cells would be needed to mimic the biochemistry of living cells. However, only a… read more here.
Keywords: macromolecular crowding; oil; high macromolecular; liposomes microfluidics ... See more keywords
Monitoring Macromolecular Crowding via FRET using Wavelength-Dependent Fluorescence Lifetime Measurements
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1885
Abstract: Macromolecular crowding influences many intracellular processes such as protein folding, biochemical reactions and diffusion. Recently, a rationally designed FRET probe was developed to quantify macromolecular crowding in vitro (Boersma et al. Nat. Meth. 12:227, 2015).… read more here.
Keywords: monitoring macromolecular; fluorescence lifetime; macromolecular crowding; lifetime measurements ... See more keywords
Macromolecular crowding induces compaction and DNA binding in the disordered N-terminal domain of hUNG2.
Sign Up to like & getrecommendations! Published in 2019 at "DNA repair"
DOI: 10.1016/j.dnarep.2019.102764
Abstract: Many human DNA repair proteins have disordered domains at their N- or C-termini with poorly defined biological functions. We recently reported that the partially structured N-terminal domain (NTD) of human uracil DNA glycosylase 2 (hUNG2),… read more here.
Keywords: hung2; terminal domain; dna; domain ... See more keywords
Size-dependent macromolecular crowding effect on the thermodynamics of protein unfolding revealed at the single molecular level.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.09.029
Abstract: The real biological environment involves a high degree of complexity and the macromolecular crowder is the best candidate to somewhat mimic this. In this contribution, we have used two different sized dextrans as model crowders… read more here.
Keywords: size dependent; thermodynamics; dependent macromolecular; effect ... See more keywords
Structural, Functional, and Dynamical Responses of a Protein in a Restricted Environment Imposed by Macromolecular Crowding.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00599
Abstract: The intercellular environment is known to be very different from the environment where most of the elementary biological processes are studied in the laboratory. As a result, there was a considerable effort on cell mimicking… read more here.
Keywords: functional dynamical; structural functional; dynamical responses; environment ... See more keywords
Enzymatic Synthesis of Cellulose Oligomer Hydrogels Composed of Crystalline Nanoribbon Networks under Macromolecular Crowding Conditions
Sign Up to like & getrecommendations! Published in 2017 at "ACS Macro Letters"
DOI: 10.1021/acsmacrolett.6b00848
Abstract: Macromolecular crowding, a solution state with high macromolecular concentrations, was used to promote the crystallization-driven self-assembly of enzymatically synthesized cellulose oligomers. Cellulose oligomers were synthesized via cellodextrin phosphorylase-catalyzed enzymatic reactions in the concentrated solutions of… read more here.
Keywords: crystalline nanoribbon; cellulose oligomer; nanoribbon networks; oligomer hydrogels ... See more keywords
A molecular sensor reveals differences in macromolecular crowding between the cytoplasm and nucleoplasm.
Sign Up to like & getrecommendations! Published in 2019 at "ACS sensors"
DOI: 10.1021/acssensors.9b00569
Abstract: We describe a molecular sensor that reports, using fluorescence resonance energy transfer (FRET), on the degree of macromolecular crowding in different cellular compartments. The oligonucleotide-based sensor is sensitive to changes in the volume fraction of… read more here.
Keywords: molecular sensor; crowding cytoplasm; sensor; macromolecular crowding ... See more keywords
Macromolecular Crowding as an Intracellular Stimulus for Responsive Nanomaterials.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c03064
Abstract: Stimuli-responsive materials are exploited in biological, materials, and sensing applications. We introduce a new endogenous stimulus, biomacromolecule crowding, which we achieve by leveraging changes in thermoresponsive properties of polymers upon high concentrations of crowding agents.… read more here.
Keywords: responsive nanomaterials; stimulus responsive; macromolecular crowding; crowding intracellular ... See more keywords
Role of Macromolecular Crowding on the Intracellular Diffusion of DNA Binding Proteins
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-18933-3
Abstract: Recent experiments suggest that cellular crowding facilitates the target search dynamics of proteins on DNA, the mechanism of which is not yet known. By using large scale computer simulations, we show that two competing factors,… read more here.
Keywords: search; dna; target search; role macromolecular ... See more keywords
Entropic effect of macromolecular crowding enhances binding between nucleosome clutches in heterochromatin, but not in euchromatin
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-23753-0
Abstract: Sharp increase in macromolecular crowding induces abnormal chromatin compaction in the cell nucleus, suggesting its non-negligible impact on chromatin structure and function. However, the details of the crowding-induced chromatin compaction remain poorly understood. In this… read more here.
Keywords: density; effect macromolecular; entropic effect; nucleosome clutches ... See more keywords
Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-89987-7
Abstract: Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-characterized measurement standards for the evaluation of the nano-environment in MMC solutions are necessary. We propose to use polarization-dependent fluorescence correlation spectroscopy (Pol-FCS)… read more here.
Keywords: diffusion; evaluation; spectroscopy; nano environment ... See more keywords