Reducing the cytoplasmic volume during hand-made cloning adversely affects the developmental competence and quality, and alters relative abundance of mRNA transcripts and epigenetic status of buffalo (Bubalus bubalis) embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Animal reproduction science"
DOI: 10.1016/j.anireprosci.2019.106136
Abstract: Hand-made cloning (HMC) is a method of choice for somatic cell nuclear transfer (SCNT). There is 20% to 50% of cytoplasm lost during manual enucleation of oocytes with HMC. To compensate, two enucleated demicytoplasts, instead… read more here.
Keywords: made cloning; embryos; developmental competence; hand made ... See more keywords