Horizontal Magnetic Tweezers to Directly Measure the Force-Velocity Relationship for Multiple Kinesin Motors.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202300558
Abstract: Transport of intracellular cargo along cytoskeletal filaments is often achieved by the concerted action of multiple motor molecules. While single-molecule studies have provided profound insight into the mechano-chemical principles and force generation of individual motors,… read more here.
Keywords: motor; velocity relationship; magnetic tweezers; force ... See more keywords
Magnetic Tweezers-Based Single-Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_6
Abstract: The ability of magnetic tweezers to apply forces and measure molecular displacements has resulted in its extensive use to study the activity of enzymes involved in various aspects of nucleic acid metabolism. These studies have… read more here.
Keywords: single molecule; recq helicase; dna; interaction ... See more keywords
Probing Chromatin Structure with Magnetic Tweezers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8591-3_18
Abstract: Magnetic tweezers form a unique tool to study the topology and mechanical properties of chromatin fibers. Chromatin is a complex of DNA and proteins that folds the DNA in such a way that meter-long stretches… read more here.
Keywords: chromatin; chromatin fibers; spectroscopy; structure ... See more keywords
Closed-loop Control Using High Power Hexapole Magnetic Tweezers for 3D Micromanipulation
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Bionic Engineering"
DOI: 10.1007/s42235-020-0009-4
Abstract: This paper presents the design, modeling, integration, and application of 3D printed high power hexapole magnetic tweezers for 3D micromanipulation applications. Six sharp-tipped magnetic poles were configured with electromagnetic coils and mounted on 3D printed… read more here.
Keywords: high power; hexapole magnetic; control; magnetic tweezers ... See more keywords
Integration of magnetic tweezers and traction force microscopy for the exploration of matrix rheology and keratinocyte mechanobiology: Model force- and displacement-controlled experiments
Sign Up to like & getrecommendations! Published in 2021 at "AIP Advances"
DOI: 10.1063/5.0041262
Abstract: In this work, we present a new experimental methodology that integrates magnetic tweezers (MT) with substrate deformation tracking microscopy (DTM) and traction force microscopy (TFM). Two types of MT-DTM/TFM experiments are described: force-control mode and… read more here.
Keywords: force displacement; microscopy; rheology; force ... See more keywords
Direct Measurement of the Stall Torque of the Flagellar Motor in Escherichia coli with Magnetic Tweezers
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.00782-22
Abstract: The maximum torque the bacterial flagellar motor generates, the stall torque, is a critical parameter that describes the motor energetics. As the motor operates in equilibrium near stall, from the stall torque one can determine… read more here.
Keywords: stall torque; flagellar motor; motor; magnetic tweezers ... See more keywords
High-resolution Single-molecule Magnetic Tweezers.
Sign Up to like & getrecommendations! Published in 2022 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-032620-104637
Abstract: Single-molecule magnetic tweezers deliver magnetic force and torque to single target molecules, permitting the study of dynamic changes in biomolecular structures and their interactions. Because the magnetic tweezer setups can generate magnetic fields that vary… read more here.
Keywords: high resolution; molecule; molecule magnetic; biochemistry ... See more keywords
3D-printed magnetic tweezers for dorsal traction force measurement.
Sign Up to like & getrecommendations! Published in 2018 at "BioTechniques"
DOI: 10.2144/btn-2018-0078
Abstract: In this study, economic magnetic tweezers (EMT) with a sharp gradient field were designed and built, in order to facilitate accurate force measurement. Our design costs less than 40 USD and is easy to mount… read more here.
Keywords: traction; magnetic tweezers; force measurement; dorsal traction ... See more keywords
Magnetic tweezers: development and use in single-molecule research.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2021-0104
Abstract: The use of magnetic tweezers for single-molecule micromanipulation has evolved rapidly since its introduction approximately 30 years ago. Magnetic tweezers have provided important insights into the dynamic activity of DNA-processing enzymes, as well as detailed, high-resolution information… read more here.
Keywords: single molecule; tweezers development; development use; magnetic tweezers ... See more keywords