Characterization of Post-Meiotic Male Germ Cell Genome Organizational States.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8663-7_16
Abstract: Dramatic and unique genome reorganizations accompany the differentiation of haploid male germ cells, characterized by a gradual loss of the vast majority of histones leading to a final tight compaction of the genome by protamines.… read more here.
Keywords: post meiotic; organizational states; characterization post; male germ ... See more keywords
Isolation of the male germ unit: organization and function in tobacco (Nicotiana tabacum L.)
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell Reports"
DOI: 10.1007/s002990050547
Abstract: Abstract Sperm cells are released from pollen tubes of tobacco as linked cells, associated with the vegetative nucleus in an assemblage known as the male germ unit (MGU). Using light microscopy, the MGU assemblage appears… read more here.
Keywords: tobacco; microscopy; sperm cells; germ unit ... See more keywords
Analysis of mouse male germ cell-specific or -predominant Tex13 family genes encoding proteins with transcriptional repressor activity
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Biology Reports"
DOI: 10.1007/s11033-021-06265-5
Abstract: Mammalian spermatogenesis is a highly organized process with successive mitotic, meiotic, and postmeiotic phases. This unique developmental process is characterized by the involvement of spermatogenic cell-specific genes. In this study, we identified and investigated testis… read more here.
Keywords: repressor activity; transcriptional repressor; male germ; activity ... See more keywords
In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells.
Sign Up to like & getrecommendations! Published in 2021 at "Cell stem cell"
DOI: 10.1016/j.stem.2021.08.005
Abstract: Mammalian male germ-cell development consists of three distinct phases: primordial germ cell (PGC) development, male germ-cell specification for spermatogonium development, and ensuing spermatogenesis. Here, we show an in vitro reconstitution of whole male germ-cell development by… read more here.
Keywords: germ cell; male germ; stem; cell ... See more keywords
CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-94851-9
Abstract: Male germ cells undergo complex developmental processes eventually producing spermatozoa through spermatogenesis, although the molecular mechanisms remain largely elusive. We have previously identified somatic cell nuclear transfer-reprogramming resistant genes (SRRGs) that are highly enriched for… read more here.
Keywords: genetic screen; reprogramming resistant; mice; male germ ... See more keywords
COP9 signalosome complex subunit 5, an IFT20 binding partner, is essential to maintain male germ cell survival and acrosome biogenesis†
Sign Up to like & getrecommendations! Published in 2019 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioz154
Abstract: Abstract Intraflagellar transport protein 20 (IFT20) is essential for spermatogenesis in mice. We discovered that COPS5 was a major binding partner of IFT20. COPS5 is the fifth component of the constitutive photomorphogenic-9 signalosome (COP9), which… read more here.
Keywords: cops5; germ; germ cell; binding partner ... See more keywords
Modern tools applied to classic structures: Approaches for mammalian male germ cell RNA granule research.
Sign Up to like & getrecommendations! Published in 2022 at "Andrology"
DOI: 10.1111/andr.13320
Abstract: : First reported in the 1800s, germ cell granules are small non-membrane bound RNA-rich regions of the cytoplasm. These sites of critical RNA processing and storage in the male germ cell are essential for proper… read more here.
Keywords: germ cell; cell; male germ; granule research ... See more keywords
Transgenerational impact of grand-paternal lifetime exposures to both folic acid deficiency and supplementation on genome-wide DNA methylation in male germ cells.
Sign Up to like & getrecommendations! Published in 2023 at "Andrology"
DOI: 10.1111/andr.13399
Abstract: BACKGROUND DNA methylation (DNAme) erasure and reacquisition occurs during prenatal male germ cell development; some further remodelling takes place after birth during spermatogenesis. Environmental insults during germline epigenetic reprogramming may affect DNAme, presenting a potential… read more here.
Keywords: methylation; germ; folic acid; dname ... See more keywords
Small RNAs on the move in male germ cells
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DOI: 10.1126/science.abj5020
Abstract: DNA in plant male meiocytes is methylated by intercellular small interfering RNAs Plants produce 24-nucleotide (nt) small interfering RNAs (siRNAs) to maintain asymmetric DNA methylation in cis—that is, an siRNA will methylate DNA in the… read more here.
Keywords: move male; rnas move; dna; germ cells ... See more keywords
MicroRNAs control mRNA fate by compartmentalization based on 3′ UTR length in male germ cells
Sign Up to like & getrecommendations! Published in 2017 at "Genome Biology"
DOI: 10.1186/s13059-017-1243-x
Abstract: BackgroundPost-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3′ UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents… read more here.
Keywords: utr length; compartmentalization; control mrna; male germ ... See more keywords
BMP4 promotes mouse iPS cell differentiation to male germ cells via Smad1/5, Gata4, Id1 and Id2.
Sign Up to like & getrecommendations! Published in 2017 at "Reproduction"
DOI: 10.1530/rep-16-0292
Abstract: Generation of male germ cells from pluripotent cells could provide male gametes for treating male infertility and offer an ideal model for unveiling molecular mechanisms of spermatogenesis. However, the influence and exact molecular mechanisms, especially… read more here.
Keywords: mouse ips; ips cells; germ cells; germ cell ... See more keywords