Improved Performance in Mammalian Cell Perfusion Cultures by Growth Inhibition
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Journal"
DOI: 10.1002/biot.201700722
Abstract: Mammalian cell perfusion cultures represent a promising alternative to the current fed‐batch technology for the production of various biopharmaceuticals. Long‐term operation at a fixed viable cell density (VCD) requires a viable culture and a constant… read more here.
Keywords: cell; mammalian cell; growth; cell perfusion ... See more keywords
Whole‐cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26254
Abstract: Many high‐value added recombinant proteins, such as therapeutic glycoproteins, are produced using mammalian cell cultures. In order to optimize the productivity of these cultures it is important to monitor cellular metabolism, for example the utilization… read more here.
Keywords: whole cell; biosensor; mammalian cell; lactate ... See more keywords
A New Lineage of Artificial Luciferases for Mammalian Cell Imaging.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_5
Abstract: The present protocol introduces a new lineage of artificial luciferases (ALucs) with unique optical properties for mammalian cell imaging. The primary candidate sequence was first created with a sequence logo generator, resulting in a total… read more here.
Keywords: cell imaging; lineage; lineage artificial; mammalian cell ... See more keywords
Applications of off-gas mass spectrometry in fed-batch mammalian cell culture
Sign Up to like & getrecommendations! Published in 2019 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-019-02242-2
Abstract: Off-gas analysis using a magnetic sector mass spectrometer was performed in mammalian cell cultures in the fed-batch mode at the 5 L bench and 50 L pilot scales. Factors affecting the MS gas traces were identified… read more here.
Keywords: mammalian cell; mass; gas; fed batch ... See more keywords
Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-017-2689-8
Abstract: The rapid growth of global biopharmaceutical market in the recent years has been a good indication of its significance in biotechnology industry. During a long period of time in recombinant protein production from 1980s, optimizations… read more here.
Keywords: engineering; line engineering; cell line; mammalian cell ... See more keywords
Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2020.107552
Abstract: The manufacturing of recombinant protein is traditionally undertaken in mammalian cell culture. Today, speed, cost and safety are the primary considerations for process improvements in both upstream and downstream manufacturing. Leaders in the biopharmaceutical industry… read more here.
Keywords: cell culture; mammalian cell; culture production; recombinant ... See more keywords
Comparative mammalian cell cytotoxicity of wastewater with elevated bromide and iodide after chlorination, chloramination, or ozonation.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of environmental sciences"
DOI: 10.1016/j.jes.2017.03.030
Abstract: Recycling wastewater is becoming more common as communities around the world try to better control their water resources against an increased frequency of either prolonged droughts or intense flooding. For communities in coastal areas, wastewaters… read more here.
Keywords: chlorination; mammalian cell; cell cytotoxicity; wastewater ... See more keywords
Development of conventional and real time PCR assays for rapid species authentication of mammalian cell lines commonly used in veterinary diagnostic laboratories.
Sign Up to like & getrecommendations! Published in 2019 at "Research in veterinary science"
DOI: 10.1016/j.rvsc.2019.08.029
Abstract: Mammalian cell lines are valuable tools in biomedical fields, with applications ranging from disease diagnosis to the production of biological reagents and vaccines. Here we report the development of new conventional (cPCR) and real time… read more here.
Keywords: real time; cell lines; mammalian cell; time pcr ... See more keywords
Mitigating Clonal Variation in Recombinant Mammalian Cell Lines.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2019.02.007
Abstract: Mammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled… read more here.
Keywords: cell lines; mammalian cell; clonal variation; cell ... See more keywords
Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines.
Sign Up to like & getrecommendations! Published in 2017 at "Virology"
DOI: 10.1016/j.virol.2017.09.025
Abstract: Sf-rhabdovirus was only recently identified as an adventitious agent of Spodoptera frugiperda (Sf) cell lines used as hosts for baculovirus vectors. As such, we still know little about its genetic variation, infectivity, and the potential… read more here.
Keywords: rhabdovirus; cell lines; mammalian cell; infectivity ... See more keywords
How to improve the dosing of chemicals in high-throughput in vitro mammalian cell assays.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical research in toxicology"
DOI: 10.1021/acs.chemrestox.9b00167
Abstract: Controlling the exposure of chemicals in in vitro mammalian cell assays is an important prerequisite for the application of in vitro methods in risk and hazard assessment of chemicals. Existing models require numerous physicochemical and… read more here.
Keywords: improve dosing; mammalian cell; cell assays; vitro ... See more keywords