Synthetic mRNA‐Based Systems in Mammalian Cells
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Biosystems"
DOI: 10.1002/adbi.201900247
Abstract: Living organisms are programmed to perform multiple functions by sensing intra‐ and extra‐cellular environments and by controlling gene expressions. Synthetic biologists aim to program cells by mimicking, designing, and constructing genetic circuits. Synthetic mRNA‐based genetic… read more here.
Keywords: based systems; synthetic mrna; mrna based; systems mammalian ... See more keywords
A Decade of Advances in Single-Cell Nanocoating for Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202100347
Abstract: Strategic advances in the single-cell nanocoating of mammalian cells have noticeably been made during the last decade, and many potential applications have been demonstrated. Various cell-coating strategies have been proposed via adaptation of reported methods… read more here.
Keywords: single cell; decade; nanocoating mammalian; mammalian cells ... See more keywords
Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Esterified Trehalose Analogues Protect Mammalian Cells from Heat Shock
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700302
Abstract: Trehalose is a disaccharide produced by many organisms to better enable them to survive environmental stresses, including heat, cold, desiccation, and reactive oxygen species. Mammalian cells do not naturally biosynthesize trehalose; however, when introduced into… read more here.
Keywords: trehalose analogues; esterified trehalose; heat shock; mammalian cells ... See more keywords
Evaluation of the mutagenic effects of Molnupiravir and N4‐hydroxycytidine in bacterial and mammalian cells by HiFi sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Environmental and Molecular Mutagenesis"
DOI: 10.1002/em.22510
Abstract: Molnupiravir (MOV) is used to treat COVID‐19. In cells, MOV is converted to the ribonucleoside analog N4‐hydroxycytidine (NHC) and incorporated into the SARS‐CoV‐2 RNA genome during its replication, resulting in RNA mutations. The widespread accumulation… read more here.
Keywords: molnupiravir hydroxycytidine; mutagenic effects; effects molnupiravir; evaluation mutagenic ... See more keywords
Glyoxylate reductase/hydroxypyruvate reductase regulates the free d-aspartate level in mammalian cells.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of cellular biochemistry"
DOI: 10.1002/jcb.30110
Abstract: Multiple d-amino acids are present in mammalian cells, and these compounds have distinctive physiological functions. Among the free d-amino acids identified in mammals, d-aspartate plays critical roles in the neuroendocrine and endocrine systems, as well… read more here.
Keywords: aspartate; reductase; reductase hydroxypyruvate; mammalian cells ... See more keywords
A new functional role of mitochondria-anchored protein ligase in peroxisome morphology in mammalian cells.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of cellular biochemistry"
DOI: 10.1002/jcb.30114
Abstract: Mitochondria and peroxisomes are metabolically interconnected and functionally active subcellular organelles. These two dynamic organelles, share a number of common biochemical functions such as β-oxidation of fatty acids and detoxification of peroxides. The biogenesis and… read more here.
Keywords: mapl; fission; mitochondria anchored; morphology ... See more keywords
Surface-Initiated Synthesis of Cell-Specific Glycopolymers using Live Mammalian Cells as Templates.
Sign Up to like & getrecommendations! Published in 2023 at "Macromolecular rapid communications"
DOI: 10.1002/marc.202200881
Abstract: Molecular recognition is an important process in life activities where specificity is the key. However, the method to gain specificity are often complex and time-consuming. Here, we have developed a novel, versatile and effective way… read more here.
Keywords: cell; initiated synthesis; mammalian cells; specific glycosurfaces ... See more keywords
Methods for Detection of Autophagy in Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/7651_2018_190
Abstract: Autophagy is a conserved catabolic process that degrades cytoplasmic constituents in the lysosome and thus contributes to the maintenance of intracellular homeostasis. The process of autophagy has been involved in many physiological and pathological processes.… read more here.
Keywords: methods detection; microscopy; detection autophagy; process ... See more keywords
Metabolomic Profiling of Adherent Mammalian Cells In Situ by LAESI-MS with Ion Mobility Separation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0030-6_15
Abstract: Ambient ionization-based mass spectrometry (MS) methods coupled with ion mobility separation (IMS) have emerged as promising approaches for high-throughput in situ analysis for biomedical to environmental applications. These methods are capable of direct profiling and… read more here.
Keywords: mobility separation; ion mobility; mammalian cells; adherent mammalian ... See more keywords
Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_22
Abstract: Bioluminescence resonance energy transfer (BRET) is a commonly used assay system for studying protein-protein interactions. The present protocol introduces a conceptually unique ligand-activatable BRET system (termed BRET9), where a full-length artificial luciferase variant 23 (ALuc23),… read more here.
Keywords: protein; system; activatable bret9; mammalian cells ... See more keywords