Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0783-1_20
Abstract: Two basic methods for the laboratory-focused cryopreservation of mammalian oocytes are described, based on work with murine oocytes. One method uses a relatively low concentration of the cryoprotectant propanediol plus sucrose and requires controlled rate… read more here.
Keywords: method; cryopreservation mammalian; vitrification; slow cooling ... See more keywords
Single-nucleus Hi-C of mammalian oocytes and zygotes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.032
Abstract: The 3D folding of the genome is linked to essential nuclear processes including gene expression, DNA repair, and replication. Chromatin conformation capture assays such as Hi-C are providing unprecedented insights into higher-order chromatin structure. Bulk… read more here.
Keywords: single nucleus; nucleus mammalian; chromatin; oocytes zygotes ... See more keywords
Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.036
Abstract: Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple… read more here.
Keywords: electrical assisted; oocytes early; microinjection; early embryos ... See more keywords
Early and later studies on action potential and fertilization potential of echinoderm oocytes and Ca2+ response of mammalian oocytes.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2019.03.002
Abstract: This is a personal essay starting from the early study on fertilization signals in echinoderm and mammalian oocytes. It presents actual examples showing that a unexpected discovery leads to unimaginable development of the research in… read more here.
Keywords: later studies; fertilization; mammalian oocytes; action potential ... See more keywords
SGK1 is essential for meiotic resumption in mammalian oocytes.
Sign Up to like & getrecommendations! Published in 2022 at "European journal of cell biology"
DOI: 10.1016/j.ejcb.2022.151210
Abstract: In mammalian females, oocytes are stored in the ovary and meiosis is arrested at the diplotene stage of prophase I. When females reach puberty oocytes are selectively recruited in cycles to grow, overcome the meiotic… read more here.
Keywords: sgk1 essential; essential meiotic; resumption; meiotic resumption ... See more keywords
The nucleolus-like and precursor bodies of mammalian oocytes and embryos and their possible role in post-fertilization centromere remodelling.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Society transactions"
DOI: 10.1042/bst20190847
Abstract: In nearly all somatic cells, the ribosome biosynthesis is a key activity. The same is true also for mammalian oocytes and early embryos. This activity is intimately linked to the most prominent nuclear organelles -… read more here.
Keywords: fertilization; oocytes embryos; nucleolus like; precursor bodies ... See more keywords
De novo programming: establishment of epigenome in mammalian oocytes
Sign Up to like & getrecommendations! Published in 2022 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioac091
Abstract: Abstract Innovations in ultrasensitive and single-cell measurements enable us to study layers of genome regulation in view of cellular and regulatory heterogeneity. Genome-scale mapping allows to evaluate epigenetic features and dynamics in different genomic contexts,… read more here.
Keywords: novo programming; mammalian oocytes; dna methylation; epigenome mammalian ... See more keywords
Molecular Mechanisms of Prophase I Meiotic Arrest Maintenance and Meiotic Resumption in Mammalian Oocytes
Sign Up to like & getrecommendations! Published in 2019 at "Reproductive Sciences"
DOI: 10.1177/1933719118765974
Abstract: Mechanisms of meiotic prophase I arrest maintenance (germinal vesicle [GV] stage) and meiotic resumption (germinal vesicle breakdown [GVBD] stage) in mammalian oocytes seem to be very complicated. These processes are regulated via multiple molecular cascades… read more here.
Keywords: arrest maintenance; mammalian oocytes; meiotic resumption; resumption ... See more keywords
Homeostatic regulation of lipid droplet content in mammalian oocytes and embryos.
Sign Up to like & getrecommendations! Published in 2021 at "Reproduction"
DOI: 10.1530/rep-21-0238
Abstract: Lipid droplets (LDs) consist of a core of neutral lipids such as triacylglycerols and cholesteryl esters covered by a phospholipid monolayer. Recent studies have shown that LDs not only store neutral lipids but are also… read more here.
Keywords: regulation lipid; content; embryonic development; mammalian oocytes ... See more keywords
Regulation of Meiotic Prophase One in Mammalian Oocytes
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2021.667306
Abstract: In female mammals, meiotic prophase one begins during fetal development. Oocytes transition through the prophase one substages consisting of leptotene, zygotene, and pachytene, and are finally arrested at the diplotene substage, for months in mice… read more here.
Keywords: one mammalian; meiotic prophase; prophase; regulation meiotic ... See more keywords
ProTAME Arrest in Mammalian Oocytes and Embryos Does Not Require Spindle Assembly Checkpoint Activity
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20184537
Abstract: In both mitosis and meiosis, metaphase to anaphase transition requires the activity of a ubiquitin ligase known as anaphase promoting complex/cyclosome (APC/C). The activation of APC/C in metaphase is under the control of the checkpoint… read more here.
Keywords: spindle assembly; oocytes embryos; mammalian oocytes; checkpoint ... See more keywords