Genetic manipulation and targeted protein degradation in mammalian systems: practical considerations, tips and tricks for discovery research.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS open bio"
DOI: 10.1002/2211-5463.13581
Abstract: Gaining a mechanistic understanding of the molecular pathways underpinning cellular and organismal physiology invariably relies on perturbation of an experimental system to infer causality. This can be achieved either by genetic manipulation or by pharmacological… read more here.
Keywords: mammalian systems; genetic manipulation; targeted protein; manipulation ... See more keywords
Transcriptional regulatory networks of the proteasome in mammalian systems
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DOI: 10.1002/iub.2586
Abstract: The tight regulation of proteostasis is essential for physiological cellular function. Mammalian cells possess a network of mechanisms that ensure proteome integrity under normal or stress conditions. The proteasome, being the major cellular proteolytic machinery,… read more here.
Keywords: proteasome mammalian; networks proteasome; regulatory networks; mammalian systems ... See more keywords
Mini-review highlighting toxic effects of triflumuron in insects and mammalian systems.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of biochemical and molecular toxicology"
DOI: 10.1002/jbt.23341
Abstract: Pesticides have been used to kill pests such as insects, fungi, rodents, and unwanted plants. As these compounds are potentially toxic to the target organisms, they could also be harmful to human health and the… read more here.
Keywords: mammalian systems; highlighting toxic; insects; mini review ... See more keywords
Targeting RNA in mammalian systems with small molecules
Sign Up to like & getrecommendations! Published in 2018 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1477
Abstract: The recognition of RNA functions beyond canonical protein synthesis has challenged the central dogma of molecular biology. Indeed, RNA is now known to directly regulate many important cellular processes, including transcription, splicing, translation, and epigenetic… read more here.
Keywords: targeting rna; mammalian systems; rna; small molecules ... See more keywords
Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems
Sign Up to like & getrecommendations! Published in 2018 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-018-2810-3
Abstract: CRISPR/Cpf1 features a number of properties that are distinct from CRISPR/Cas9 and provides an excellent alternative to Cas9 for genome editing. To date, genome engineering by CRISPR/Cpf1 has been reported only in human cells and… read more here.
Keywords: genome editing; system; mammalian systems; crispr cpf1 ... See more keywords
A novel mammalian glucokinase exhibiting exclusive inorganic polyphosphate dependence in the cell nucleus
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2017.09.004
Abstract: Background Hexokinase and glucokinase enzymes are ubiquitously expressed and use ATP and ADP as substrates in mammalian systems and a variety of polyphosphate substrates and/or ATP in some eukaryotic and microbial systems. Polyphosphate synthesising or… read more here.
Keywords: mammalian systems; glucokinase; glucokinase exhibiting; novel mammalian ... See more keywords
How Cells Deal with the Fluctuating Environment: Autophagy Regulation under Stress in Yeast and Mammalian Systems
Sign Up to like & getrecommendations! Published in 2022 at "Antioxidants"
DOI: 10.3390/antiox11020304
Abstract: Eukaryotic cells frequently experience fluctuations of the external and internal environments, such as changes in nutrient, energy and oxygen sources, and protein folding status, which, after reaching a particular threshold, become a type of stress.… read more here.
Keywords: mammalian systems; stress; stress yeast; autophagy regulation ... See more keywords