Reporter gene knock-in into Marc-145 cells using CRISPR/Cas9-mediated homologous recombination
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Letters"
DOI: 10.1007/s10529-020-02860-x
Abstract: Objectives Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign… read more here.
Keywords: 145 cells; site; marc 145; gene ... See more keywords
Overexpression of RACK1 enhanced the replication of porcine reproductive and respiratory syndrome virus in Marc-145 cells and promoted the NF-κB activation via upregulating the expression and phosphorylation of TRAF2.
Sign Up to like & getrecommendations! Published in 2019 at "Gene"
DOI: 10.1016/j.gene.2019.05.046
Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative pathogen for porcine reproductive and respiratory syndrome (PRRS), which lead to huge loss to porcine industry. RACK1 (receptor of activated protein C kinase 1) was… read more here.
Keywords: rack1; 145 cells; porcine reproductive; activation ... See more keywords
A Nanobody Targeting Viral Nonstructural Protein 9 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Virology"
DOI: 10.1128/jvi.01888-18
Abstract: The pandemic outbreak of PRRS, which is caused by PRRSV, has greatly affected the swine industry. We still lack an efficient vaccine, and it is an immense challenge to control its infection. An intracellularly expressed… read more here.
Keywords: protein; nanobody; 145 cells; tat nb6 ... See more keywords
Porcine reproductive and respiratory syndrome virus inhibits MARC-145 proliferation via inducing apoptosis and G2/M arrest by activation of Chk/Cdc25C and p53/p21 pathway
Sign Up to like & getrecommendations! Published in 2018 at "Virology Journal"
DOI: 10.1186/s12985-018-1081-9
Abstract: Porcine reproductive and respiratory syndrome virus(PRRSV) is an important immunosuppressive virus which can suppresses infected cells proliferation. In this work, we examined PRRSV ability to manipulate cell cycle progression of MARC-145 cells and explored the… read more here.
Keywords: respiratory syndrome; porcine reproductive; arrest; reproductive respiratory ... See more keywords
Porcine Interferon Stimulated Gene 12a Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication in MARC-145 Cells
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms18081613
Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe losses in the global pig industry. In the present study, we investigated the molecular characterization of porcine interferon stimulated gene 12a (ISG12A) and confirmed its anti-PRRSV… read more here.
Keywords: prrsv; 145 cells; porcine reproductive; marc 145 ... See more keywords
A Triple Amino Acid Substitution at Position 88/94/95 in Glycoprotein GP2a of Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV1) Is Responsible for Adaptation to MARC-145 Cells
Sign Up to like & getrecommendations! Published in 2019 at "Viruses"
DOI: 10.3390/v11010036
Abstract: The Meat Animal Research Center-145 (MARC-145) cell line has been proven to be valuable for viral attenuation regarding vaccine development and production. Cell-adaptation is necessary for the efficient replication of porcine reproductive and respiratory syndrome… read more here.
Keywords: amino acid; 145 cells; porcine reproductive; adaptation ... See more keywords