CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200088
Abstract: The chloroplast represents an attractive compartment for light‐driven biosynthesis of recombinant products, and advanced synthetic biology tools are available for engineering the chloroplast genome ( = plastome) of several algal and plant species. However, producing… read more here.
Keywords: strategy; cpposneg positive; marker free; selection ... See more keywords
Marker-free transgenic rice plant overexpressing pea LecRLK imparts salinity tolerance by inhibiting sodium accumulation
Sign Up to like & getrecommendations! Published in 2018 at "Plant Molecular Biology"
DOI: 10.1007/s11103-018-0816-8
Abstract: Key messagePsLecRLK overexpression in rice provides tolerance against salinity stress and cause upregulation of SOS1 pathway genes, which are responsible for extrusion of excess Na+ ion under stress condition.AbstractSoil salinity is one of the most… read more here.
Keywords: plant; rice; tolerance; marker free ... See more keywords
Object extraction via deep learning-based marker-free tracking framework of surgical instruments for laparoscope-holder robots
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Computer Assisted Radiology and Surgery"
DOI: 10.1007/s11548-020-02214-y
Abstract: Purpose The surgical instrument tracking framework, especially the marker-free surgical instrument tracking framework, is the key to visual servoing which is applied to achieve active control for laparoscope-holder robots. This paper presented a marker-free surgical… read more here.
Keywords: framework; marker free; tracking framework; free tracking ... See more keywords
Selectable Markers to Marker-Free Selection in Rice
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biotechnology"
DOI: 10.1007/s12033-022-00460-w
Abstract: Inadequate rice production worldwide is largely attributed to abiotic and biotic stresses, along with high sensitivity of cultivable plant germplasm. In the field of cereal biotechnology, rice engineering plays an important role in achieving tolerance… read more here.
Keywords: free selection; rice engineering; marker free; selection ... See more keywords
Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.02.068
Abstract: SUMMARY Genome editing technologies have transformed our ability to engineer desired genomic changes within living systems. However, detecting precise genomic modifications often requires sophisticated, expensive, and time-consuming experimental approaches. Here, we describe DTECT (Dinucleotide signaTurE… read more here.
Keywords: genome editing; capture; detection; marker free ... See more keywords
Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation
Sign Up to like & getrecommendations! Published in 2017 at "Crop Journal"
DOI: 10.1016/j.cj.2017.04.001
Abstract: Abstract Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergence of transgenic technology has enabled improvements in the drought resistance of rice plants and helped avert crop damage… read more here.
Keywords: rice; drought; drought tolerance; marker free ... See more keywords
Joint Iterative Fast Projection Matching for Fully Automatic Marker-free Alignment of Nano-tomography Reconstructions
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-62949-1
Abstract: Highly accurate, fully automatic marker-free image alignment plays an important role in nano-tomographic reconstruction, particularly in cases where the spatial resolution of the tomographic system is on the nanometer scale. However, highly accurate marker-free methods… read more here.
Keywords: fully automatic; alignment; marker free; projection matching ... See more keywords
Enhancing trehalose biosynthesis improves yield potential in marker-free transgenic rice under drought, saline, and sodic conditions
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Experimental Botany"
DOI: 10.1093/jxb/erz462
Abstract: Marker-free transgenic lines of rice are developed with enhanced trehalose accumulation that is associated with improved grain yield under salinity, sodicity, and drought stresses. read more here.
Keywords: rice; free transgenic; drought; marker free ... See more keywords
Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template
Sign Up to like & getrecommendations! Published in 2022 at "Plant Physiology"
DOI: 10.1093/plphys/kiac075
Abstract: Abstract Gene targeting (GT) for precise gene insertion or swap into pre-defined genomic location has been a bottleneck for expedited soybean precision breeding. We report a robust selectable marker-free GT system in soybean, one of… read more here.
Keywords: marker; gene targeting; ochrobactrum haywardense; gene ... See more keywords
A selection marker-free method for gene deletion and editing in baculovirus genomes.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of virological methods"
DOI: 10.2139/ssrn.4154109
Abstract: Here, we develop a simple, efficient, bacmid-based, selection marker-free method for gene deletion and editing in baculovirus genomes. Specifically, based on pFastbac1, a donor plasmid with long left and right homology arms but without a… read more here.
Keywords: baculovirus; selection marker; free method; method ... See more keywords
Video-based marker-free tracking and multi-scale analysis of mouse locomotor activity and behavioral aspects in an open field arena: A perspective approach to the quantification of complex gait disturbances associated with Alzheimer's disease
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Neuroinformatics"
DOI: 10.3389/fninf.2023.1101112
Abstract: Introduction Complex gait disturbances represent one of the prominent manifestations of various neurophysiological conditions, including widespread neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Therefore, instrumental measurement techniques and automatic computerized analysis appears essential for… read more here.
Keywords: gait disturbances; analysis; complex gait; approach ... See more keywords