Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector
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DOI: 10.1007/s00253-018-9496-1
Abstract: Selectable marker recycling is a basic technique in bioengineering. However, this technique is usually unavailable in non-model microorganisms. In this study, we proposed a simple and efficient method for selectable marker recycling in the astaxanthin-synthesizing… read more here.
Keywords: vector; cre; yeast; selectable marker ... See more keywords
Marker Recycling in Candida albicans through CRISPR-Cas9-Induced Marker Excision
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DOI: 10.1128/msphere.00050-17
Abstract: It is critical to be able to alter genes in order to elucidate their functions. These alterations often rely upon markers that allow selection for a rare cell in a population that has incorporated a… read more here.
Keywords: crispr cas9; selection; marker; cas9 induced ... See more keywords