Expression of Alternative Nitrogenases in Rhodopseudomonas palustris Is Enhanced Using an Optimized Genetic Toolset for Rapid, Markerless Modifications.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.0c00496
Abstract: The phototrophic bacterium Rhodopseudomonas palustris is emerging as a promising biotechnological chassis organism, due to its resilience to a range of harsh conditions, a wide metabolic repertoire, and the ability to quickly regenerate ATP using… read more here.
Keywords: markerless; overexpression; alternative nitrogenases; rhodopseudomonas palustris ... See more keywords
Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac649
Abstract: Abstract Despite advances in bacterial genome engineering, delivery of large synthetic constructs remains challenging in practice. In this study, we propose a straightforward and robust approach for the markerless integration of DNA fragments encoding whole… read more here.
Keywords: counterselection; markerless; integration; robust counterselection ... See more keywords
Markerless deletion of putative alanine dehydrogenase genes in Bacillus licheniformis using a codBA-based counterselection technique.
Sign Up to like & getrecommendations! Published in 2017 at "Microbiology"
DOI: 10.1099/mic.0.000544
Abstract: Bacillus licheniformis strains are used for the large-scale production of industrial exoenzymes from proteinaceous substrates, but details of the amino acid metabolism involved are largely unknown. In this study, two chromosomal genes putatively involved in… read more here.
Keywords: alanine; markerless; bacillus licheniformis; counterselection ... See more keywords
Validation of a 3D Markerless System for Gait Analysis Based on OpenPose and Two RGB Webcams
Sign Up to like & getrecommendations! Published in 2021 at "IEEE Sensors Journal"
DOI: 10.1109/jsen.2021.3081188
Abstract: Markerless human motion capture could be a potential tool for studying body biomechanics. Despite the extensive previous studies, the design of an accurate markerless system able to perform a quantitative clinical gait analysis is still… read more here.
Keywords: markerless; system; gait analysis; markerless system ... See more keywords
The reliability and validity of gait analysis system using 3D markerless pose estimation algorithms
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.857975
Abstract: Quantifying kinematic gait for elderly people is a key factor for consideration in evaluating their overall health. However, gait analysis is often performed in the laboratory using optical sensors combined with reflective markers, which may… read more here.
Keywords: markerless; gait analysis; reliability; markerless pose ... See more keywords
Comparison of Lower Extremity Joint Moment and Power Estimated by Markerless and Marker-Based Systems during Treadmill Running
Sign Up to like & getrecommendations! Published in 2022 at "Bioengineering"
DOI: 10.3390/bioengineering9100574
Abstract: Background: Markerless (ML) motion capture systems have recently become available for biomechanics applications. Evidence has indicated the potential feasibility of using an ML system to analyze lower extremity kinematics. However, no research has examined ML… read more here.
Keywords: system; markerless; lower extremity; moment ... See more keywords