Articles with "markerless deletions" as a keyword



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An efficient blue-white screening system for markerless deletions and stable integrations in Streptomyces chromosomes based on the blue pigment indigoidine biosynthetic gene bpsA

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Published in 2018 at "Applied Microbiology and Biotechnology"

DOI: 10.1007/s00253-018-9393-7

Abstract: We previously developed an efficient deletion system for streptomycetes based on the positive selection of double-crossover events using bpsA, a gene for producing the blue pigment indigoidine. Using this system, we removed interfering secondary metabolite… read more here.

Keywords: system; markerless deletions; pigment indigoidine; blue pigment ... See more keywords