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Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02093-21
Abstract: Methanocaldococcus has been considered a model genus, but genetic tools have not been forthcoming until recently. Here, we develop and illustrate the utility of positive selection with either of two selective agents (simvastatin and neomycin),…
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Keywords:
deletion;
markerless frame;
hyperthermophilic methanogen;
genetic tools ... See more keywords