Development of a dual-antimicrobial counterselection method for markerless genetic engineering of bacterial genomes
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DOI: 10.1007/s00253-018-9565-5
Abstract: Markerless genetic engineering of bacterial genomes is commonly performed by two-step homologous recombination methods using vectors carrying flanking regions of the target gene for site-specific vector integration and counterselection markers to provide positive selection pressure… read more here.
Keywords: markerless genetic; method; selection; counterselection ... See more keywords