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   Articles with "mass photometry" as a keyword



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Mass Photometry of Membrane Proteins

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recommendations! Published in 2021 at "Chem"

DOI: 10.1016/j.chempr.2020.11.011

Abstract: Summary Integral membrane proteins (IMPs) are biologically highly significant but challenging to study because they require maintaining a cellular lipid-like environment. Here, we explore the application of mass photometry (MP) to IMPs and membrane-mimetic systems… read more here.

Keywords: mass photometry; photometry membrane; membrane proteins; microscopy ... See more keywords

Quantifying the heterogeneity of macromolecular machines by mass photometry

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recommendations! Published in 2020 at "Nature Communications"

DOI: 10.1038/s41467-020-15642-w

Abstract: Sample purity is central to in vitro studies of protein function and regulation, and to the efficiency and success of structural studies using techniques such as x-ray crystallography and cryo-electron microscopy (cryo-EM). Here, we show… read more here.

Keywords: mass photometry; quantifying heterogeneity; mass; microscopy ... See more keywords

Surface passivation and functionalisation for mass photometry

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recommendations! Published in 2024 at "Journal of Microscopy"

DOI: 10.1111/jmi.13302

Abstract: Interferometric scattering (iSCAT) microscopy enables the label‐free observation of biomolecules. Consequently, single‐particle imaging and tracking with the iSCAT‐based method known as mass photometry (MP) is a growing area of study. However, establishing reliable cover glass… read more here.

Keywords: passivation functionalisation; mass photometry; surface; functionalisation ... See more keywords

Engineering Protein–Peptide Interfaces via Combinatorial Mutagenesis and Mass Photometric Screening

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recommendations! Published in 2025 at "Biomolecules"

DOI: 10.3390/biom15081183

Abstract: The SpyTag–SpyCatcher system, developed by the Howarth lab, is based on splitting the CnaB2 domain from Streptococcus pyogenes into two parts: a 13-amino-acid SpyTag and a 116-amino-acid SpyCatcher. Upon incubation, they spontaneously form a covalent… read more here.

Keywords: spycatcher; mass photometry; mutagenesis; spytag ... See more keywords

Automated Mass Photometry of Adeno-Associated Virus Vectors from Crude Cell Extracts

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recommendations! Published in 2024 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms25020838

Abstract: Mass photometry (MP) is a fast and simple analysis method for the determination of the proportions of subpopulations in an AAV sample. It is label-free and requires minimal sample volumes between 5–10 µL, which makes… read more here.

Keywords: vectors crude; mass photometry; mass; cell extracts ... See more keywords