Mass spectrometry‐based abundance atlas of ABC transporters in human liver, gut, kidney, brain and skin
Sign Up to like & getrecommendations! Published in 2020 at "Febs Letters"
DOI: 10.1002/1873-3468.13982
Abstract: ABC transporters (ATP‐binding cassette transporter) traffic drugs and their metabolites across membranes, making ABC transporter expression levels a key factor regulating local drug concentrations in different tissues and individuals. Yet, quantification of ABC transporters remains… read more here.
Keywords: abc transporters; mass spectrometry; abundance; brain ... See more keywords
In situ glucosylceramide synthesis and its pharmacological inhibition analysed in cells by 13C5‐sphingosine precursor feeding and mass spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14448
Abstract: Glycosphingolipids (GSLs) fulfil diverse functions in cells. Abnormalities in their metabolism are associated with specific pathologies and, consequently, the pharmacological modulation of GSLs is considered a therapeutic avenue. The accurate measurement of in situ metabolism of… read more here.
Keywords: mass spectrometry; precursor; situ glucosylceramide; synthesis ... See more keywords
Emerging Computational Methods in Mass Spectrometry Imaging
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203339
Abstract: Mass spectrometry imaging (MSI) is a powerful analytical technique that generates maps of hundreds of molecules in biological samples with high sensitivity and molecular specificity. Advanced MSI platforms with capability of high‐spatial resolution and high‐throughput… read more here.
Keywords: mass spectrometry; computational methods; emerging computational; spectrometry imaging ... See more keywords
Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Accelerated Workflow for Evaluation of Lead Monoclonal Antibodies.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology journal"
DOI: 10.1002/biot.202100358
Abstract: BACKGROUND Epitope mapping is an increasingly important aspect of biotherapeutic and vaccine development. Recent advances in therapeutic antibody design and production have enabled candidate mAbs to be identified at a rapidly increasing rate, resulting in… read more here.
Keywords: mass spectrometry; exchange mass; epitope screening; hydrogen deuterium ... See more keywords
Mapping the Interactions of Selective Biochemical Probes of Antibody Conformation by Hydrogen–Deuterium Exchange Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201600670
Abstract: Protein‐based pharmaceuticals represent the fastest growing group of drugs in development in the pharmaceutical industry. One of the major challenges in the discovery, development, and distribution of biopharmaceuticals is the assessment of changes in their… read more here.
Keywords: mass spectrometry; exchange mass; antibody; hydrogen deuterium ... See more keywords
Mitochondrion‐Targeting Identification of a Fluorescent Apoptosis‐Triggering Molecule by Mass Spectrometry Elucidates Drug Tracking
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800598
Abstract: The real‐time tracking of localization and dynamics of small molecules in organelles helps to understand their function and identification of their potential targets at subcellular resolution. To identify the mitochondrion‐targeting effects of small molecules (NA‐17… read more here.
Keywords: mitochondrion; identification; mitochondrion targeting; mass spectrometry ... See more keywords
Automatic Full Glycan Structural Determination through Logically Derived Sequence Tandem Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201900228
Abstract: Glycans have diverse functions and play vital roles in many biological systems, such as influenza, vaccines, and cancer biomarkers. However, full structural identification of glycans remains challenging. The glycan structure was conventionally determined by chemical… read more here.
Keywords: tandem mass; mass; mass spectrometry; structural determination ... See more keywords
Rapid, Label‐Free Screening of Diverse Biotransformations by Flow‐Injection Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202300170
Abstract: Mass spectrometry‐based high‐throughput screening methods combine the advantages of photometric or fluorometric assays and analytical chromatography, as they are reasonably fast (throughput ≥1 sample/min) and broadly applicable, with no need for labelled substrates or products.… read more here.
Keywords: biotransformations flow; mass spectrometry; flow injection; mass ... See more keywords
Coherent Point Drift Peak Alignment Algorithms Using Distance and Similarity Measures for Two-Dimensional Gas Chromatography Mass Spectrometry Data.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of chemometrics"
DOI: 10.1002/cem.3236
Abstract: The peak alignment is a vital preprocessing step before downstream analysis, such as biomarker discovery and pathway analysis, for two-dimensional gas chromatography mass spectrometry (2DGCMS)-based metabolomics data. Due to uncontrollable experimental conditions, e.g., the differences… read more here.
Keywords: mass spectrometry; algorithms; peak alignment; distance ... See more keywords
Native Mass Spectrometry for the Study of PROTAC GNE‐987‐Containing Ternary Complexes
Sign Up to like & getrecommendations! Published in 2021 at "Chemmedchem"
DOI: 10.1002/cmdc.202100113
Abstract: PROteolysis TArgeting Chimeras (PROTACs) promote the degradation, rather than inhibition, of a drug target as a mechanism for therapeutic treatment. Bifunctional PROTAC molecules allow simultaneous binding of both the target protein and an E3‐Ubiquitin ligase,… read more here.
Keywords: gne 987; native mass; ternary complexes; mass spectrometry ... See more keywords
Desorption Electrospray Ionization Mass Spectrometry Assay for Label‐Free Characterization of SULT2B1b Enzyme Kinetics
Sign Up to like & getrecommendations! Published in 2022 at "ChemMedChem"
DOI: 10.1002/cmdc.202200043
Abstract: The sulfotransferase (SULT) 2B1b, which catalyzes the sulfonation of 3β‐hydroxysteroids, has been identified as a potential target for prostate cancer treatment. However, a major limitation for SULT2B1b‐targeted drug discovery is the lack of robust assays… read more here.
Keywords: label free; sult2b1b; mass spectrometry; desorption electrospray ... See more keywords