A Large Stokes Shift Fluorescent Protein Constructed from the Fusion of Red Fluorescent mCherry and Far‐Red Fluorescent BDFP1.6
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800695
Abstract: Phycobiliproteins are constituents of phycobilisomes that can harvest orange, red, and far‐red light for photosynthesis in cyanobacteria and red algae. Phycobiliproteins in the phycobilisome cores, such as allophycocyanins, absorb far‐red light to funnel energy to… read more here.
Keywords: large stokes; red fluorescent; fluorescent protein; mcherry ... See more keywords
Immunohistochemical amplification of mCherry fusion protein is necessary for proper visualization
Sign Up to like & getrecommendations! Published in 2020 at "MethodsX"
DOI: 10.1016/j.mex.2020.100946
Abstract: Highlights • Virally expressed fluorescent reporter mCherry fusion protein does not adequately demonstrate its presence in fixed tissue.• Immunohistochemical amplification of mCherry can rescue this deficit. read more here.
Keywords: amplification mcherry; immunohistochemical amplification; mcherry fusion; mcherry ... See more keywords
Detection of Histidine-Tagged Protein in Escherichia coli by Single-Cell Inductively Coupled Plasma-Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00774
Abstract: We have developed a rapid and precise quantification method for a histidine (His)-tagged recombinant protein produced in Escherichia coli (E. coli) by single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). Plasmid vector containing enhanced green fluorescent protein… read more here.
Keywords: escherichia coli; single cell; mcherry; coli single ... See more keywords
Fast Purification of Recombinant Monomeric Amyloid-β from E. coli and Amyloid-β-mCherry Aggregates from Mammalian Cells
Sign Up to like & getrecommendations! Published in 2020 at "ACS Chemical Neuroscience"
DOI: 10.1021/acschemneuro.0c00300
Abstract: The Alzheimer’s disease related peptide, Amyloid-beta (Aβ)1–40 and 1–42, has proven difficult to be purified as a recombinant monomeric protein due its expression in E. coli leading to the formation of insoluble inclusion bodies and… read more here.
Keywords: amyloid; recombinant monomeric; mcherry; mammalian cells ... See more keywords
Optimization of Electrotransformation Parameters and Engineered Promoters for Lactobacillus plantarum from Wine.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00123
Abstract: Robust and versatile promoters for Lactobacillus plantarum found in wine are necessary gene expression tools for genetic research involving wine stress. We optimized the electrotransformation parameters for L. plantarum XJ25 isolated from wine and engineered… read more here.
Keywords: promoters lactobacillus; wine; electrotransformation; mcherry ... See more keywords
Histological assessment of optogenetic tools to study fronto-visual and fronto-parietal cortical networks in the rhesus macaque
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-67752-6
Abstract: Optogenetics offers unprecedented possibilities to investigate cortical networks. Yet, the number of successful optogenetic applications in non-human primates is still low, and the consequences of opsin expression in the primate brain are not well documented.… read more here.
Keywords: cortical networks; chr2 eyfp; enphr3 mcherry; histological assessment ... See more keywords
Study of the persistence and dynamics of recombinant mCherry‐producing Yarrowia lipolytica strains in the mouse intestine using fluorescence imaging
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14178
Abstract: Yarrowia lipolytica is a dimorphic oleaginous non‐conventional yeast widely used as a powerful host for expressing heterologous proteins, as well as a promising source of engineered cell factories for various applications. This microorganism has a… read more here.
Keywords: yarrowia lipolytica; lipolytica; mcherry; study persistence ... See more keywords
Construction of an Integrated mCherry Red Fluorescent Protein Expression System for Labeling and Tracing in Lactiplantibacillus plantarum WCFS1
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.690270
Abstract: Thorough intestinal adhesion and colonization greatly promote the probiotic properties of lactic acid bacteria (LAB). Labeling and tracing with fluorescent proteins are effective and reliable for studying the in vivo physiological activities of LAB including… read more here.
Keywords: protein; system; fluorescent; wcfs1 ... See more keywords
Structural Insights into the Binding of Red Fluorescent Protein mCherry-Specific Nanobodies
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24086952
Abstract: Red fluorescent proteins (RFPs) have broad applications in life science research, and the manipulation of RFPs using nanobodies can expand their potential uses. However, the structural information available for nanobodies that bind with RFPs is… read more here.
Keywords: lam8; red fluorescent; structural insights; mcherry ... See more keywords