Quantitative measurement of intracellular HDAC1/2 drug occupancy using a trans-cyclooctene largazole thiol probe.
Sign Up to like & getrecommendations! Published in 2017 at "MedChemComm"
DOI: 10.1039/c6md00633g
Abstract: Histone deacetylases (HDACs) regulate diverse cellular processes, and are promising targets for a number of diseases. Here we describe the design and utilization of a largazole-based chemical probe to quantitatively measure the intracellular occupancy of… read more here.
Keywords: quantitative measurement; probe; measurement intracellular; drug ... See more keywords
Seeing through cells: Rapid measurement of intracellular target proteins
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.996224
Abstract: We have studied a method for making microbial cells transparent by immersing them in a solution with a high refractive index (RI). When the RI of the solution was matching that of the cells, light… read more here.
Keywords: cells rapid; measurement intracellular; seeing cells; target proteins ... See more keywords