Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs).
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1843-1_6
Abstract: Model lipid bilayers tethered to a gold substrate with molecular tethers are constructed. The conductance versus temperature dependence curve is then obtained. Here, a method to measure the activation energy for translocation of an ion… read more here.
Keywords: lipid membranes; measuring activation; tethered bilayer; bilayer lipid ... See more keywords
A FRET-based biosensor for measuring Gα13 activation in single cells
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DOI: 10.1371/journal.pone.0193705
Abstract: Förster Resonance Energy Transfer (FRET) provides a way to directly observe the activation of heterotrimeric G-proteins by G-protein coupled receptors (GPCRs). To this end, FRET based biosensors are made, employing heterotrimeric G-protein subunits tagged with… read more here.
Keywords: based biosensor; measuring activation; fret based; activation ... See more keywords