Paramagnetic 1H NMR Spectroscopy to Investigate the Catalytic Mechanism of Radical S-Adenosylmethionine Enzymes.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2019.08.018
Abstract: Iron-sulfur clusters in radical S-adenosylmethionine (SAM) enzymes catalyze an astonishing array of complex and chemically challenging reactions across all domains of life. Here we showed that 1H NMR spectroscopy experiments tailored to reveal hyperfine-shifted signals… read more here.
Keywords: radical adenosylmethionine; nmr spectroscopy; sam; mechanism radical ... See more keywords
Mechanism of Radical Formation in the H-Bond Network of D1-Asn298 in Photosystem II.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00574
Abstract: In photosystem II (PSII), redox-active tyrosine Z (TyrZ) forms a low-barrier H-bond with Nε of D1-His190. The PSII crystal structures show that Nδ of D1-His190 donates an H-bond to the carbonyl O of D1-Asn298. However,… read more here.
Keywords: asn298; bond; photosystem; mechanism radical ... See more keywords
Mechanism of Radical Initiation in the Radical SAM Enzyme Superfamily.
Sign Up to like & getrecommendations! Published in 2023 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-052621-090638
Abstract: Radical S-adenosylmethionine (SAM) enzymes use a site-differentiated [4Fe-4S] cluster and SAM to initiate radical reactions through liberation of the 5'-deoxyadenosyl (5'-dAdo•) radical. They form the largest enzyme superfamily, with more than 700,000 unique sequences currently, and… read more here.
Keywords: radical sam; sam; enzyme superfamily; mechanism radical ... See more keywords