Nucleic acid duplexes with metal-mediated base pairs and their structures
Sign Up to like & getrecommendations! Published in 2019 at "Coordination Chemistry Reviews"
DOI: 10.1016/j.ccr.2019.05.007
Abstract: Abstract Metal-mediated base pairs represent a topical area of research at the border of bioinorganic chemistry, supramolecular coordination chemistry and DNA nanotechnology. In a metal-mediated base pair, the hydrogen bonds of a canonical base pair… read more here.
Keywords: metal mediated; mediated base; base; chemistry ... See more keywords
Light-induced formation of silver(I)-mediated base pairs in DNA: Possibilities and limitations
Sign Up to like & getrecommendations! Published in 2020 at "Inorganica Chimica Acta"
DOI: 10.1016/j.ica.2020.119856
Abstract: Abstract The applicability of the caged nucleoside CNPP (2′-deoxycytidine bearing an ortho-nitrophenylpropyl group at the exocyclic N4 position) in the light-triggered formation of three silver(I)-mediated base pairs was probed, namely C–Ag(I)–C, Im–Ag(I)–C and P–Ag(I)–C (Im = imidazole… read more here.
Keywords: mediated base; silver mediated; formation; base pairs ... See more keywords
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.09.029
Abstract: The past several years have seen an explosion in development of applications for the CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to recruitment of a range of DNA and chromatin-modifying enzymes. While homology-directed… read more here.
Keywords: base editing; mediated base; methods applications; crispr mediated ... See more keywords
CRISPR–Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development
Sign Up to like & getrecommendations! Published in 2018 at "Nature Cell Biology"
DOI: 10.1038/s41556-018-0202-4
Abstract: CRISPR-mediated base editing can introduce single-nucleotide changes in the DNA of living cells. One intriguing application of base editing is to screen pivotal amino acids for protein function in vivo; however, it has not been… read more here.
Keywords: base editing; mediated base; amino acids; base ... See more keywords
Benzothiazole-substituted 1,3-diaza-2-oxophenoxazine as a luminescent nucleobase surrogate for silver(I)-mediated base pairing.
Sign Up to like & getrecommendations! Published in 2022 at "Dalton transactions"
DOI: 10.1039/d2dt01762h
Abstract: A benzothiazole-substituted derivative (X) of 1,3-diaza-2-oxophenoxazine was evaluated with respect to its ability to engage in Ag(I)-mediated homo base pair formation in two different DNA duplexes. The metal binding was determined by a combination of… read more here.
Keywords: mediated base; spectroscopy; base pair; benzothiazole substituted ... See more keywords
beditor: A Computational Workflow for Designing Libraries of Guide RNAs for CRISPR-Mediated Base Editing
Sign Up to like & getrecommendations! Published in 2019 at "Genetics"
DOI: 10.1534/genetics.119.302089
Abstract: The biological relevance of the present method pertains to CRISPR-mediated base editing technology that has opened new avenues for scar-free genome-wide mutagenesis and thus to a wide range of applications in genome editing... CRISPR-mediated base… read more here.
Keywords: base editing; mediated base; guide rnas; crispr mediated ... See more keywords