Homology-mediated end joining-based targeted integration using CRISPR/Cas9
Sign Up to like & getrecommendations! Published in 2017 at "Cell Research"
DOI: 10.1038/cr.2017.76
Abstract: Targeted integration of transgenes can be achieved by strategies based on homologous recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end joining (NHEJ). The more generally used HR is inefficient for achieving gene integration in… read more here.
Keywords: end joining; mediated end; integration; homology ... See more keywords
POLθ-mediated end joining is restricted by RAD52 and BRCA2 until the onset of mitosis.
Sign Up to like & getrecommendations! Published in 2021 at "Nature cell biology"
DOI: 10.1038/s41556-021-00764-0
Abstract: BRCA2-mutant cells are defective in homologous recombination, making them vulnerable to the inactivation of other pathways for the repair of DNA double-strand breaks (DSBs). This concept can be clinically exploited but is currently limited due… read more here.
Keywords: end joining; mitosis; mediated end; brca2 ... See more keywords