Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2018 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-018-2537-1
Abstract: Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly… read more here.
Keywords: mediated genome; crispr cas9; cas9 mediated; genome editing ... See more keywords
‘Artificial spermatid'-mediated genome editingâ€
Sign Up to like & getrecommendations! Published in 2019 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioz087
Abstract: Abstract For years, extensive efforts have been made to use mammalian sperm as the mediator to generate genetically modified animals; however, the strategy of sperm-mediated gene transfer (SMGT) is unable to produce stable and diversified… read more here.
Keywords: genome editing; haescs; spermatid mediated; mediated genome ... See more keywords
CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
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DOI: 10.1186/s13059-017-1237-8
Abstract: CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example,… read more here.
Keywords: genome editing; crispr; crispr cas9; exon skipping ... See more keywords
CRISPR/Cas9-mediated genome editing in vancomycin-producing strain Amycolatopsis keratiniphila
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1141176
Abstract: Amycolatopsis is an important source of diverse valuable bioactive natural products. The CRISPR/Cas-mediated gene editing tool has been established in some Amycolatopsis species and has accomplished the deletion of single gene or two genes. The… read more here.
Keywords: genome editing; crispr cas9; eco 0501; amycolatopsis ... See more keywords