Overcoming the Limitations of Sortase with Proximity-Based Sortase-Mediated Ligation (PBSL).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9537-0_13
Abstract: S. aureus sortase A (SrtA), a calcium-dependent bacterial transpeptidase, is commonly used to site-specifically label proteins containing a LPXTG SrtA recognition motif with a wide array of chemical moieties. A major limitation of sortase-mediated labeling,… read more here.
Keywords: sortase; mediated ligation; proximity based; sortase mediated ... See more keywords
Selective Enrichment and Quantification of N-Terminal Glycine Peptides via Sortase A Mediated Ligation.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b03562
Abstract: The identification and quantification of low-abundant proteins are always impeded by high-abundant proteins in proteomic analysis because of the extreme complexity of peptide mixtures and wide dynamic range of protein abundances. Here, we developed a… read more here.
Keywords: mediated ligation; glycine peptides; sortase mediated; terminal glycine ... See more keywords
Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag.
Sign Up to like & getrecommendations! Published in 2020 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.0c00156
Abstract: Sortase-mediated ligation is a powerful method for generating site-specifically modified proteins. However, this process is limited by the inherent reversibility of the ligation reaction. To address this, here we report the continued development and optimization… read more here.
Keywords: mediated ligation; efficient sortase; ligation; sortase mediated ... See more keywords