An enzyme-mediated protein-fragment complementation assay for substrate screening of sortase A.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.03.016
Abstract: Enzyme-mediated protein conjugation has gained great attention recently due to the remarkable site-selectivity and mild reaction condition affected by the nature of enzyme. Among all sorts of enzymes reported, sortase A from Staphylococcus aureus (SaSrtA)… read more here.
Keywords: fragment complementation; protein fragment; enzyme; enzyme mediated ... See more keywords
Electrostatically Mediated Protein-Protein Interactions for Monoclonal Antibodies: A Combined Experimental and Coarse-Grained Molecular Modeling Approach.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2018.11.004
Abstract: Electrostatically mediated protein-protein interactions (PPI) can influence key product properties such as solubility, solution viscosity, and aggregation rates. Predictive models would allow for candidates/formulations to be screened with little or no protein material. Three monoclonal… read more here.
Keywords: protein interactions; monoclonal antibodies; electrostatically mediated; protein protein ... See more keywords
Generic Plug-and-Play Strategy for High-Throughput Analysis of PTM-Mediated Protein Complexes.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00521
Abstract: Protein complexes mediated by various post-translational modifications (PTMs) play important roles in almost every aspect of biological processes. PTM-mediated protein complexes often have weak and transient binding properties, which limit their unbiased profiling especially in… read more here.
Keywords: protein complexes; mediated protein; domain; plug play ... See more keywords
Asparaginyl Endopeptidase-Mediated Protein C-Terminal Hydrazinolysis for the Synthesis of Bioconjugates.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.1c00551
Abstract: Asparaginyl endopeptidases (AEPs) are cysteinyl enzymes naturally catalyzing the hydrolysis and transpeptidation reactions at Asx-Xaa bonds. These reactions go by a common acyl-enzyme thioester intermediate, which is either attacked by water (for a protease-AEP) or… read more here.
Keywords: endopeptidase mediated; protein terminal; terminal hydrazinolysis; mediated protein ... See more keywords
Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16299-1
Abstract: Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively… read more here.
Keywords: protein; intein mediated; protein interactions; protein protein ... See more keywords
Design of metal-mediated protein assemblies via hydroxamic acid functionalities.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00535-z
Abstract: The self-assembly of proteins into sophisticated multicomponent assemblies is a hallmark of all living systems and has spawned extensive efforts in the construction of novel synthetic protein architectures with emergent functional properties. Protein assemblies in… read more here.
Keywords: protein; construction; protein assemblies; metal ... See more keywords
SecA-Mediated Protein Translocation through the SecYEG Channel.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiology spectrum"
DOI: 10.1128/microbiolspec.psib-0028-2019
Abstract: In bacteria, the Sec translocase mediates the translocation of proteins into and across the cytoplasmic membrane. It consists of a protein conducting channel SecYEG, the ATP-dependent motor SecA, and the accessory SecDF complex. Here we… read more here.
Keywords: translocation secyeg; mediated protein; translocation; protein translocation ... See more keywords
Systematic identification of phosphorylation-mediated protein interaction switches
Sign Up to like & getrecommendations! Published in 2017 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1005462
Abstract: Proteomics techniques can identify thousands of phosphorylation sites in a single experiment, the majority of which are new and lack precise information about function or molecular mechanism. Here we present a fast method to predict… read more here.
Keywords: phosphorylation mediated; systematic identification; phosphorylation sites; identification phosphorylation ... See more keywords
Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.934179
Abstract: Polydopamine (PDA) is a biocompatible cell-adhesive polymer with versatile applications in biomedical devices. Previous studies have shown that PDA coating could improve cell adhesion and differentiation of human mesenchymal stem cells (hMSCs). However, there is… read more here.
Keywords: protein adsorption; mediated protein; epigenetic status; differentiation ... See more keywords
Intein-Mediated Protein Engineering for Cell-Based Biosensors
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12050283
Abstract: Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors… read more here.
Keywords: cell based; cell; mediated protein; protein engineering ... See more keywords
In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe
Sign Up to like & getrecommendations! Published in 2021 at "Molecules"
DOI: 10.3390/molecules26144273
Abstract: Protein folding is important for protein homeostasis/proteostasis in the human body. We have established the ability to manipulate protein unfolding/refolding for β-lactoglobulin using the induced mechanical energy in the thin film microfluidic vortex fluidic device… read more here.
Keywords: protein; using aggregation; aggregation induced; vortex fluidic ... See more keywords