Agrobacterium-Mediated Transformation of Leaf Base Segments.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6682-0_7
Abstract: Agrobacterium-mediated transformation has become a routine method of genetic engineering of cereals, gradually replacing the biolistic protocols. Simple integration patterns of transgenic loci, decent transformation efficiency, and technical simplicity are the main advantages offered by… read more here.
Keywords: transformation leaf; agrobacterium mediated; transformation; leaf base ... See more keywords
Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7874-8_8
Abstract: Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency… read more here.
Keywords: mediated transformation; transformation efficiency; agrobacterium mediated; transformation ... See more keywords
Ensifer-Mediated Transformation (EMT) of Rice (Monocot) and Oilseed Rape (Dicot).
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8778-8_3
Abstract: Ensifer adhaerens OV14 underpins the successful crop transformation protocol, termed Ensifer-mediated transformation (EMT). The adaptability and efficiency of EMT technology to successfully transform both monocot and dicots have been previously reported. To facilitate community users'… read more here.
Keywords: emt; rice; oilseed rape; transformation ... See more keywords
Agrobacterium tumefaciens-mediated transformation of oleaginous yeast Lipomyces species
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8357-7
Abstract: Interest in using renewable sources of carbon, especially lignocellulosic biomass, for the production of hydrocarbon fuels and chemicals has fueled interest in exploring various organisms capable of producing hydrocarbon biofuels and chemicals or their precursors.… read more here.
Keywords: lipomyces species; yeast lipomyces; transformation; biology ... See more keywords
A tau class GST, OsGSTU5, interacts with VirE2 and modulates the Agrobacterium-mediated transformation in rice.
Sign Up to like & getrecommendations! Published in 2022 at "Plant cell reports"
DOI: 10.1007/s00299-021-02824-z
Abstract: KEY MESSAGE OsGSTU5 interacts and glutathionylates the VirE2 protein of Agrobacterium and its (OsGSTU5) overexpression and downregulation showed a low and high AMT efficiency in rice, respectively. During Agrobacterium-mediated transformation (AMT), T-DNA along with several… read more here.
Keywords: mediated transformation; vire2; agrobacterium mediated; rice ... See more keywords
Agrobacterium tumefaciens-mediated transformation as an efficient tool for insertional mutagenesis of Cercospora zeae-maydis.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2016.12.010
Abstract: An efficient Agrobacterium tumefaciens-mediated transformation (ATMT) approach was developed for the plant pathogenic fungus, Cercospora zeae-maydis, which is the causative agent of gray leaf spot in maize. The transformation was evaluated with five parameters to… read more here.
Keywords: zeae maydis; transformation; mediated transformation; agrobacterium tumefaciens ... See more keywords
A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as effective gene targeting tools for Aspergillus carbonarius.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.01.015
Abstract: In recent years, versatile genetic tools have been developed and applied to a number of filamentous fungi of industrial importance. However, the existing techniques have limitations when it comes to achieve the desired genetic modifications,… read more here.
Keywords: agrobacterium; gene targeting; aspergillus carbonarius; gene ... See more keywords
Development of an A. tumefaciens-mediated transformation system for Tilletia controversa Kühn.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106313
Abstract: Dwarf bunt of wheat caused by Tilletia controversa Kühn has been identified an international quarantine disease, which replace the grain material into millions of teliospores. A. tumefaciens-mediated transformation (ATMT) system is a powerful tool for… read more here.
Keywords: controversa; system; tilletia controversa; transformation ... See more keywords
Elemental Sulfur-Mediated Transformation of Carboxylic Acids to Acyl Fluorides by Electrophilic Fluorinating Reagent, Selectfluor.
Sign Up to like & getrecommendations! Published in 2023 at "Organic letters"
DOI: 10.1021/acs.orglett.3c00701
Abstract: The elemental sulfur-mediated synthesis of acyl fluorides from carboxylic acids is achieved using Selectfluor. A broad range of acyl fluorides are accessible from carboxylic acids while avoiding the formation of acid anhydrides. 19F NMR spectra… read more here.
Keywords: sulfur mediated; selectfluor; acyl fluorides; mediated transformation ... See more keywords
Unreported cytochalasins from an acid-mediated transformation of cytochalasin J isolated from Diaporthe cf. ueckeri.
Sign Up to like & getrecommendations! Published in 2023 at "Fitoterapia"
DOI: 10.2139/ssrn.4291227
Abstract: Chemical investigation of an endophytic fungus herein identified as Diaporthe cf. ueckeri yielded four known compounds, named cytochalasins H and J and dicerandrols A and B. Reports of acid sensitivity within the cytochalasan family, inspired… read more here.
Keywords: unreported cytochalasins; cytochalasins acid; acid mediated; diaporthe ueckeri ... See more keywords
Development of Genetic Tools in Glucoamylase-Hyperproducing Industrial Aspergillus niger Strains
Sign Up to like & getrecommendations! Published in 2022 at "Biology"
DOI: 10.3390/biology11101396
Abstract: Simple Summary Glucoamylase is one of the most needed industrial enzymes in the food and biofuel industries. Aspergillus niger is a commonly used cell factory for the production of commercial glucoamylase. For decades, genetic manipulation… read more here.
Keywords: aspergillus niger; genetic tools; glucoamylase; mediated transformation ... See more keywords