Integration of cell permeabilization and medium engineering for enhanced enantioselective synthesis of ethyl-S-3-hydroxy-3-phenylpropanoate (S-EHPP)
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2019.04.020
Abstract: Abstract Low permeability of microbial cells and mass transfer limitations are major problems for efficient whole-cell biocatalysis. In this work, a method for enhanced enantioselective synthesis of S-EHPP, an intermediate for antidepressant drug fluoxetine via… read more here.
Keywords: medium; integration cell; cell; medium engineering ... See more keywords
Construction of a Highly Diastereoselective Aldol Reaction System with l-Threonine Aldolase by Computer-Assisted Rational Molecular Modification and Medium Engineering.
Sign Up to like & getrecommendations! Published in 2020 at "Organic letters"
DOI: 10.1021/acs.orglett.0c01792
Abstract: Diastereoselectivity of l-threonine aldolase (LTA) was determined by paths of aldehydes attacking a pyridoxal phosphate-glycine complex. Thus, strategies of enhancing the syn path and blocking the anti path were performed to modify LTA. A mutant… read more here.
Keywords: threonine aldolase; lta; medium engineering;