Articles with "melting analysis" as a keyword



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Genotyping DNA Variants with High-Resolution Melting Analysis.

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Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6442-0_2

Abstract: High-resolution melting analysis (HRMA) is a simple, quick, and effective method to scan and screen PCR amplicons for sequence variants. HRMA is a nondestructive closed tube assay; after PCR, DNA melting can directly be performed… read more here.

Keywords: high resolution; dna; resolution melting; analysis ... See more keywords
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Genetic species identification of ecologically important planthoppers (Prokelisia spp.) of coastal Spartina saltmarshes using High Resolution Melting Analysis (HRMA)

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Published in 2019 at "Scientific Reports"

DOI: 10.1038/s41598-019-56518-4

Abstract: Phloem-feeding planthoppers of the genus Prokelisia rank among the most abundant and ecologically important browsers of coastal saltmarsh grasses of eastern North America and the Caribbean. Along the Spartina marshes of the northern Gulf of… read more here.

Keywords: ecologically important; high resolution; resolution melting; analysis hrma ... See more keywords
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TaqMan probes as blocking agents for enriched PCR amplification and DNA melting analysis of mutant genes.

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Published in 2017 at "BioTechniques"

DOI: 10.2144/000114515

Abstract: Asymmetric PCR and DNA melting analysis with TaqMan probes applied for mutation detection is effectively used in clinical diagnostics. The method is simple, cost-effective, and carried out in a closed-tube format, minimizing time, labor, and… read more here.

Keywords: dna melting; blocking agents; taqman probes; melting analysis ... See more keywords