Multicolor melting curve analysis discloses high carrier frequency of hearing loss‐associated variants among neonates in Jiangsu province
Sign Up to like & getrecommendations! Published in 2024 at "Molecular Genetics & Genomic Medicine"
DOI: 10.1002/mgg3.2384
Abstract: Genetic disorders ascribe to half of cases of congenital hearing loss. Hearing screening is significant in detecting hearing loss (HL) but weak at diagnosis, which can be complemented by genetic screening. read more here.
Keywords: loss; multicolor melting; hearing loss; curve analysis ... See more keywords
Heat transfer time determination based on DNA melting curve analysis
Sign Up to like & getrecommendations! Published in 2019 at "Microfluidics and Nanofluidics"
DOI: 10.1007/s10404-019-2308-9
Abstract: The determination of the physical properties of fluids—such as the thermal characteristics, which include heat transfer time (Δt)—is becoming more challenging as system sizes shrink to micro- and nanometer scales. Hence, knowledge of these properties… read more here.
Keywords: melting curve; analysis; transfer time; determination ... See more keywords
A novel multiplex PCR for virus detection by melting curve analysis
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Virological Methods"
DOI: 10.1016/j.jviromet.2018.09.010
Abstract: Abstract Background Rapid and accurate laboratory diagnoses of viral infections are crucial for the management and treatment of patients with viral infections. Conventional methods for virus detection are labourious, time consuming, and only a single… read more here.
Keywords: detection; virus detection; melting curve; virus ... See more keywords
High-resolution melting-curve (HRM) analysis for C. meleagridis identification in stool samples.
Sign Up to like & getrecommendations! Published in 2018 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2017.12.070
Abstract: BACKGROUND Cryptosporidiosis represents a major public health problem. This infection, caused by a protozoan parasite of the genus Cryptosporidium, has been reported worldwide as a frequent cause of diarrhoea. In the immunocompetent host, the typical… read more here.
Keywords: curve hrm; high resolution; resolution melting; melting curve ... See more keywords
High-level Multiplexing in Digital PCR With Intercalating Dyes by Coupling Real-Time Kinetics and Melting Curve Analysis.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c03298
Abstract: Digital polymerase chain reaction (dPCR) is a mature technique that has enabled scientific breakthroughs in several fields. However, this technology is primarily used in research environments with high-level multiplexing representing a major challenge. Here, we… read more here.
Keywords: melting curve; level multiplexing; high level; curve analysis ... See more keywords
RepFluo, a Fast Fluorescent In Vitro Assay of Cas9 Activity Exploiting Melting Curve Analysis
Sign Up to like & getrecommendations! Published in 2025 at "ACS Omega"
DOI: 10.1021/acsomega.5c02066
Abstract: Demand for less labor-intensive in vitro assays of the activity of CRISPR/Cas proteins is rising to extend the potential applications of CRISPR in the field of diagnostics. RNA guided DNA endonucleases of the Cas family… read more here.
Keywords: repfluo fast; analysis; curve analysis; melting curve ... See more keywords
Melting Curve and Phase Relations of Fe‐Ni Alloys: Implications for the Earth's Core Composition
Sign Up to like & getrecommendations! Published in 2020 at "Geophysical Research Letters"
DOI: 10.1029/2020gl088169
Abstract: Nickel is the second most abundant element in the Earth's core. However, the properties of Fe‐Ni alloys are still poorly constrained under planetary cores conditions, in particular concerning the effect of Ni on the melting… read more here.
Keywords: melting curve; earth core; curve; curve phase ... See more keywords
Entropy and transport properties of liquid metals along the melting curve
Sign Up to like & getrecommendations! Published in 2017 at "AIP Advances"
DOI: 10.1063/1.4976824
Abstract: Molecular dynamics simulations are performed for several monatomic metals and Fe0.9Ni0.1 metallic alloy to study the transport properties and entropy of liquids along melting curve. Our results show that the self-diffusion coefficients and viscosity of… read more here.
Keywords: melting curve; along melting; transport properties; liquid metals ... See more keywords
Detection of azole-susceptible and azole-resistant Aspergillus coinfection by cyp51A PCR amplicon melting curve analysis
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Antimicrobial Chemotherapy"
DOI: 10.1093/jac/dkx262
Abstract: Background The AsperGenius® assay is a multiplex real-time PCR test that allows the simultaneous detection of Aspergillus species and identification of the most common mutations in the Aspergillus fumigatus cyp51A gene conferring resistance (TR34/L98H and… read more here.
Keywords: detection; melting curve; azole; azole resistant ... See more keywords
Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH Technology
Sign Up to like & getrecommendations! Published in 2021 at "Open Forum Infectious Diseases"
DOI: 10.1093/ofid/ofab364
Abstract: Abstract Background The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations. Methods Here we present a cost-efficient and… read more here.
Keywords: base specific; melting curve; variants concern; concern ... See more keywords
Melting curve of vanadium up to 256 GPa: Consistency between experiments and theory
Sign Up to like & getrecommendations! Published in 2020 at "Physical Review B"
DOI: 10.1103/physrevb.102.214104
Abstract: Youjun Zhang ,1 Ye Tan ,2 Hua Y. Geng,2 Nilesh P. Salke,3 Zhipeng Gao,2 Jun Li ,2,* Toshimori Sekine,4 Qingming Wang,1 Eran Greenberg ,5,† Vitali B. Prakapenka,5 and Jung-Fu Lin 6,‡ 1Institute of Atomic and… read more here.
Keywords: melting curve; vanadium 256; physics; curve vanadium ... See more keywords