Membrane affinity and fluorescent labelling: comparative study of monolayer interaction, cellular uptake and cytotoxicity profile of carboxyfluorescein-conjugated cationic peptides
Sign Up to like & getrecommendations! Published in 2018 at "Amino Acids"
DOI: 10.1007/s00726-018-2630-7
Abstract: Fluorescent labelling is a common approach to reveal the molecular details of cellular uptake, internalisation, transport, distribution processes in biological systems. The conjugation with a fluorescent moiety might affect relevant physico-chemical and in vitro transport… read more here.
Keywords: conjugation; membrane affinity; cationic peptides; interaction ... See more keywords
Aggregation Induced Conformation Changes Determine Amylin Membrane Affinity
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1960
Abstract: Amylin is a 37 residue intrinsically disordered protein whose aggregation is associated with Type II diabetes. For most amyloid aggregates, the intermediate aggregations states (“oligomers”) are supposed to be more toxic than the mature fibrillar… read more here.
Keywords: conformation; aggregation; aggregation induced; membrane affinity ... See more keywords
Rapidly Visualizing the Membrane Affinity of Gene Vectors Using Polydiacetylene-Based Allochroic Vesicles.
Sign Up to like & getrecommendations! Published in 2019 at "ACS sensors"
DOI: 10.1021/acssensors.9b00102
Abstract: The high-throughput screening of chemically active substances has aroused widespread interest in recent years. The screening of drug carriers is usually ignored, although they interact directly with physiological barriers and target cells, and they determine… read more here.
Keywords: gene; membrane affinity; gene vectors; affinity gene ... See more keywords
Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods.
Sign Up to like & getrecommendations! Published in 2019 at "American journal of veterinary research"
DOI: 10.2460/ajvr.80.5.449
Abstract: OBJECTIVE To compare ultracentrifugation, precipitation, and membrane affinity chromatography methods for isolation of extracellular vesicles (EVs) from canine plasma samples and to identify suitable reference genes for incorporation into a quantitative reverse transcription PCR assay… read more here.
Keywords: membrane affinity; reference; ultracentrifugation precipitation; precipitation membrane ... See more keywords
Quantitative Analysis of the Membrane Affinity of Local Anesthetics Using a Model Cell Membrane
Sign Up to like & getrecommendations! Published in 2021 at "Membranes"
DOI: 10.3390/membranes11080579
Abstract: Local anesthesia is a drug that penetrates the nerve cell membrane and binds to the voltage gate sodium channel, inhibiting the membrane potential and neurotransmission. It is mainly used in clinical uses to address the… read more here.
Keywords: membrane affinity; model; membrane; cell membrane ... See more keywords