Recent advances in nanodisc technology for membrane protein studies (2012–2017)
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12706
Abstract: Historically, the main barrier to membrane protein investigations has been the tendency of membrane proteins to aggregate (due to their hydrophobic nature), in aqueous solution as well as on surfaces. The introduction of biomembrane mimetics… read more here.
Keywords: membrane proteins; microscopy; membrane protein; recent advances ... See more keywords
Cleavage of the outer membrane protein OmpX by protealysin regulates Serratia proteamaculans invasion
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Letters"
DOI: 10.1002/1873-3468.13897
Abstract: Protealysin is a thermolysin‐like protease of Serratia proteamaculans capable of specifically cleaving actin, which correlates with the invasive activity of these bacteria. Here, we show that inactivation of the protealysin gene does not inhibit invasion… read more here.
Keywords: serratia proteamaculans; protein ompx; protealysin; outer membrane ... See more keywords
Interplay between membrane proteins and membrane protein-lipid pertaining to plant salinity stress.
Sign Up to like & getrecommendations! Published in 2023 at "Cell biochemistry and function"
DOI: 10.1002/cbf.3798
Abstract: High salinity in agricultural lands is one of the predominant issues limiting agricultural yields. Plants have developed several mechanisms to withstand salinity stress, but the mechanisms are not effective enough for most crops to prevent… read more here.
Keywords: membrane proteins; membrane protein; membrane; salinity stress ... See more keywords
Foldable Detergents for Membrane Protein Stability
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200276
Abstract: Detergents are widely used for membrane protein structural study. Many recently developed detergents contain multiple tail and head groups, which are typically connected via a small and branched linker. Due to their inherent compact structures,… read more here.
Keywords: detergents membrane; alkyl chain; protein stability; membrane protein ... See more keywords
Predicted N‐terminal N‐linked glycosylation sites may underlie membrane protein expression patterns in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2021 at "Yeast"
DOI: 10.1002/yea.3657
Abstract: N‐linked glycosylation is one type of posttranslational modification that proteins undergo during expression. The following describes the effects of N‐linked glycosylation on high‐level membrane protein expression in yeast with an emphasis on Saccharomyces cerevisiae. N‐linked… read more here.
Keywords: membrane protein; linked glycosylation; expression; glycosylation ... See more keywords
Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1394-8_12
Abstract: This chapter outlines a protocol developed to prepare a purified deuterated membrane protein for a small-angle neutron scattering (SANS) experiment. SANS is a noninvasive technique well suited to studying membrane protein solution structures, and deuteration… read more here.
Keywords: protein; small angle; deuterated membrane; membrane protein ... See more keywords
Structural Stringency of Cholesterol for Membrane Protein Function Utilizing Stereoisomers as Novel Tools: A Review.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6875-6_3
Abstract: Cholesterol is an important lipid in the context of membrane protein function. The function of a number of membrane proteins, including G protein-coupled receptors (GPCRs) and ion channels, has been shown to be dependent on… read more here.
Keywords: protein function; membrane; membrane protein; cholesterol ... See more keywords
Defining Membrane Protein Topology Using pho-lac Reporter Fusions.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7033-9_10
Abstract: Experimental determination of membrane protein topology can be achieved using various techniques. Here we present the pho-lac dual reporter system, a simple, convenient, and reliable tool to analyze the topology of membrane proteins in vivo.… read more here.
Keywords: reporter; pho lac; phoa; topology ... See more keywords
Solid-State NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7151-0_18
Abstract: Structural studies of membrane proteins (MP) in a native or native-like environment remain a challenge. X-ray crystallography of three-dimensional crystals of MP in lipids and cryo-electron microscopy of two-dimensional crystals also in lipids have given… read more here.
Keywords: solid state; membrane protein; state nmr;
Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7151-0_9
Abstract: Membrane proteins are difficult to manipulate and stabilize once they have been removed from their native membranes. However, despite these difficulties, successes in membrane-protein structure determination have continued to accumulate for over two decades, thanks… read more here.
Keywords: scattering techniques; crystallization; detergent; membrane protein ... See more keywords
Analysis of Membrane Protein Topology in the Plant Secretory Pathway.
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DOI: 10.1007/978-1-4939-7262-3_7
Abstract: Topology of membrane proteins provides important information for the understanding of protein function and intermolecular associations. Integrate membrane proteins are generally transported from endoplasmic reticulum (ER) to Golgi and downstream compartments in the plant secretory… read more here.
Keywords: protein; plant secretory; topology; protein topology ... See more keywords