Recent advances in nanodisc technology for membrane protein studies (2012–2017)
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12706
Abstract: Historically, the main barrier to membrane protein investigations has been the tendency of membrane proteins to aggregate (due to their hydrophobic nature), in aqueous solution as well as on surfaces. The introduction of biomembrane mimetics… read more here.
Keywords: membrane proteins; microscopy; membrane protein; recent advances ... See more keywords
Interplay between membrane proteins and membrane protein-lipid pertaining to plant salinity stress.
Sign Up to like & getrecommendations! Published in 2023 at "Cell biochemistry and function"
DOI: 10.1002/cbf.3798
Abstract: High salinity in agricultural lands is one of the predominant issues limiting agricultural yields. Plants have developed several mechanisms to withstand salinity stress, but the mechanisms are not effective enough for most crops to prevent… read more here.
Keywords: membrane proteins; membrane protein; membrane; salinity stress ... See more keywords
Active role of glia‐like supporting cells in the organ of Corti: Membrane proteins and their roles in hearing
Sign Up to like & getrecommendations! Published in 2022 at "Glia"
DOI: 10.1002/glia.24229
Abstract: The organ of Corti, located in the cochlea in the inner ear, is one of the major sensory organs involved in hearing. The organ of Corti consists of hair cells, glia‐like supporting cells, and the… read more here.
Keywords: membrane proteins; like supporting; organ corti; glia like ... See more keywords
Quantitative Analysis of Endocytic Recycling of Membrane Proteins by Monoclonal Antibody‐Based Recycling Assays
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.25503
Abstract: In this report, we present an analysis of several recycling protocols based on labeling of membrane proteins with specific monoclonal antibodies (mAbs). We analyzed recycling of membrane proteins that are internalized by clathrin‐dependent endocytosis, represented… read more here.
Keywords: membrane proteins; analysis; antibody; recycling membrane ... See more keywords
Solution nuclear magnetic resonance spectroscopy of bacterial outer membrane proteins in natively excreted vesicles using engineered Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1302
Abstract: Gaining structural information on membrane proteins in their native lipid environment is a long‐standing challenge in molecular biology. Instead, it is common to employ membrane mimetics, which has been shown to affect protein structure, dynamics,… read more here.
Keywords: membrane proteins; outer membrane; spectroscopy; resonance ... See more keywords
Advances in mass spectrometry‐based footprinting of membrane proteins
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100222
Abstract: Structural biology is entering an exciting time where many new high‐resolution structures of large complexes and membrane proteins (MPs) are determined regularly. These advances have been driven by over 15 years of technological improvements, first… read more here.
Keywords: membrane proteins; mass spectrometry; advances mass; based footprinting ... See more keywords
Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0767-1_2
Abstract: Endocytosis and endosomal trafficking to vacuoles play important roles in regulating the homeostasis of plasma membrane (PM) proteins in plant cells. FREE1 (FYVE domain protein required for endosomal sorting 1) is a plant-unique component of… read more here.
Keywords: analysis membrane; membrane proteins; transport endosomal; endosomal compartments ... See more keywords
Exploring Phosphoinositide Binding Using Native Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1142-5_11
Abstract: Phosphoinositides interact with proteins to fulfill various functions in the cell. In many cases, they specifically recruit peripheral membrane proteins to biological membranes. The analysis of their interactions with proteins is therefore essential for understanding… read more here.
Keywords: native mass; membrane proteins; mass spectrometry; mass ... See more keywords
Single-Particle Cryo-EM of Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1394-8_9
Abstract: In the recent years, the protein databank has been fueled by the exponential growth of high-resolution electron cryo-microscopy (cryo-EM) structures. This trend will be further accelerated through the continuous software and method developments and the… read more here.
Keywords: high resolution; membrane proteins; biology; single particle ... See more keywords
Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6454-3_14
Abstract: Thirty percent of the human proteome is composed of membrane proteins that can perform a wide range of cellular functions and communications. They represent the core of modern medicine as the targets of about 50… read more here.
Keywords: membrane proteins; weak alignment; dna; nmr structure ... See more keywords
The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7151-0_16
Abstract: Membrane proteins are key cellular components that perform essential functions. They are major therapeutic targets. Electron crystallography can provide structural experimental information at atomic scale for membrane proteins forming two-dimensional (2D) crystals. There are two… read more here.
Keywords: membrane proteins; cracam robot; crystallization; two dimensional ... See more keywords